50-year-old assumptions about strength muscled aside

Doctors have a new way of thinking about how to treat heart and skeletal muscle diseases.

Body builders have a new way of thinking about how they maximize their power. Both owe their new insight to high-energy X-rays, a moth and cloud computing.

The understanding of how muscles get their power has been greatly expanded with new results published online July 10 in the Royal Society journal Proceedings of the Royal Society B. The Royal Society is the U.K.’s national academy of sciences.

The basics of how a muscle generates power remain the same: Filaments of myosin tugging on filaments of actin shorten, or contract, the muscle – but the power doesn’t just come from what’s happening straight up and down the length of the muscle, as has been assumed for 50 years.

Instead, University of Washington-led research shows that as muscles bulge, the filaments are drawn apart from each other, the myosin tugs at sharper angles over greater distances, and it’s that action that deserves credit for half the change in muscle force scientists have been measuring.

Researchers made this discovery when using computer modeling to test the geometry and physics of the 50-year-old understanding of how muscles work. The computer results of the force trends were validated through X-ray diffraction experiments on moth flight muscle, which is very similar to human cardiac muscle. The X-ray work was led by co-author Thomas Irving, an Illinois Institute of Technology professor and director of the Biophysics Collaborative Access Team (Bio-CAT) beamline at the Advanced Photon Source, which is housed at the U.S. Department of Energy’s Argonne National Laboratory.

A previous lack of readily available access to computational power and X-ray diffraction facilities are two reasons that this is the first time these findings have been documented, speculated lead-author C. David Williams, who earned his doctorate at the UW while conducting the research, and now is a postdoctoral researcher at Harvard University.  Currently, X-ray lightsources have a waiting list of about three researchers for every one active experiment. The APS is undergoing an upgrade that will greatly increase access and research power and expedite data collection.

The new understanding of muscle dynamics derived from this study has implications for the research and use of all muscles, including organs.

“In the heart especially, because the muscle surrounds the chambers that fill with blood, being able to account for forces that are generated in several directions during muscle contraction allows for much more accurate and realistic study of how pressure is generated to eject blood from the heart,” said co-author Michael Regnier, a UW bioengineering professor. “The radial and long axis forces that are generated may be differentially compromised in cardiac diseases and these new, detailed models allow this to be studied at a molecular level for the first time. They also take us to a new level in testing therapeutic treatments targeted to contractile proteins for both cardiac and skeletal muscle diseases. ”

This study gives scientists and doctors a new basis for interpreting experiments and understanding the mechanisms that regulate muscle contraction. Researchers have known for sometime that the muscle filament lattice spacing changes over the length-tension curve, but its importance in generating the steep length dependence of force has not been previously demonstrated.

“The predominant thinking of the last 50 years is that 100 percent of the muscle force comes from changes as muscles shorten and myosin and actin filaments overlap. But when we isolated the effects of filament overlap we only got about half the change in force that physiologists know muscles are capable of producing,” Williams said.

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The rest of the force, he said, should be credited to the lattice work of filaments as it expands outward in bulging muscle – whether in a body builder’s buff biceps or the calves of a sinewy marathon runner.

“One of the major discoveries that David Williams brought to light is that force is generated in multiple directions, not just along the long axis of muscle as everyone thinks, but also in the radial direction,” said Thomas Daniel, UW professor of biology and co-author on the paper.

“This aspect of muscle force generation has flown under the radar for decades and is now becoming a critical feature of our understanding of normal and pathological aspects of muscle,” Daniel added.

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