A genetic disruption strategy developed by CU Boulder researchers effectively stymies the evolution of antibiotic-resistant bacteria such as E. coli, giving scientists a crucial leg up in the ongoing battle against deadly superbugs.
These multidrug-resistant pathogens—which adapt to current antibiotics faster than new ones can be created—infect nearly 2 million people and cause at least 23,000 deaths annually in the U.S., according to data from the Centers for Disease Control.
In an effort to develop a sustainable long-term solution, CU Boulder researchers created the Controlled Hindrance of Adaptation of OrganismS (CHAOS) approach, which uses CRISPR DNA editing techniques to modify multiple gene expressions within the bacteria cells, stunting the pathogen’s central processes and thwarting its ability to evolve defenses.
The findings are outlined today in the journal Communications Biology and could open new research avenues on how to best restrict a pathogen’s antibiotic resistance.
“We now have a way to cut off the evolutionary pathways of some of the nastiest bugs and potentially prevent future bugs from emerging at all,” said Peter Otoupal, lead author of the study and a doctoral researcher in CU Boulder’s Department of Chemical and Biological Engineering (CHBE).
The CHAOS research is the culmination of work that began in 2013, when Otoupal and his colleagues began searching for genes that could act as a cellular kill switch for E. coli. When the scientists tweaked one gene at a time, the bacteria could adapt and survive. But when they altered two or more genes at once, the cell got weaker.
“We saw that when we tweaked multiple gene expressions at the same time—even genes that would seemingly help the bacteria survive—the bacteria’s fitness dropped dramatically,” Otoupal said.
The CHAOS method takes advantage of this effect, pulling multiple genetic levers in order to build up stress on the bacterial cell and eventually trigger a cascading failure, leaving the bug more vulnerable to current treatments. The technique does not alter the bug’s DNA itself, only the expression of individual genes, similar to the way a coded message is rendered useless without the proper decryption.
“You can think of it in terms of a series of escalating annoyances to the cell that eventually cause it to weaken,” said Anushree Chatterjee, senior author of the study and an assistant professor in CHBE. “This method offers tremendous potential to create more effective combinatorial approaches.”
Although E. coli has nearly 4,000 individual genes, the exact gene modification sequence appears to matter less than the sheer number of genes that are disrupted, Otoupal said. Still, the researchers plan to continue optimizing the CHAOS method to seek out the most efficient disruptions.“Diseases are very dynamic, so we need to design smarter therapies that can gain control over their rapid adaptation rates,” Chatterjee said. “The emphasis in our lab is demonstrating the efficacy of these methods and then finding ways to translate the technology to modern clinical settings.”
“In the past, nobody really considered that it might be possible to slow down evolution,” Otoupal said. “But like anything else, evolution has rules and we’re starting to learn how to use them to our advantage.”
Learn more: How to stop an antibiotic-resistant superbug
The Latest on: Genetic disruption
[google_news title=”” keyword=”genetic disruption” num_posts=”10″ blurb_length=”0″ show_thumb=”left”]
via Google News
The Latest on: Genetic disruption
- British girl’s hearing restored after ground-breaking gene therapyon May 9, 2024 at 6:08 pm
LONDON: An 18-month-old British girl, born completely deaf, is believed to be the youngest person in history to have had their hearing restored. The girl underwent a ground-breaking new form of gene ...
- Deaf baby hears for the first time after ‘groundbreaking’ gene therapy trialon May 9, 2024 at 10:22 am
Opal Sandy, an 18-month-old from England, is the youngest child to come close to “normal hearing levels” in a gene therapy trial to aid her genetic deafness.
- UK girl's hearing restored after groundbreaking gene therapyon May 9, 2024 at 5:41 am
An 18-month old British girl who was born completely deaf is believed to be the youngest person to have their hearing restored after undergoing groundbreaking new gene therapy.
- Deaf girl is cured in world first gene therapy trialon May 8, 2024 at 11:32 pm
Opal Sandy, aged 18 months, was born completely deaf due to condition auditory neuropathy ...
- Home-based cancer care gaining momentum after the pandemic disruption: Experton May 8, 2024 at 7:30 pm
Home-based cancer care gaining momentum after the pandemic disruption: Expert: Shardul Nautiyal, Mumbai Thursday, May 9, 2024, 08:00 Hrs [IST] Home-based cancer care is one of the ...
- CRISPR Enzyme Found in Metagenomic Study Is Tiny, Yet Active and Preciseon May 6, 2024 at 6:30 pm
Metagenomic study uncovers previously uncharacterized Cas9 nucleases, including CoCas9, which is compact, but still active and precise.
- Imagine living in a 4-foot body that doesn't develop chronic diseaseson April 30, 2024 at 9:04 am
That genetic disruption blocks the body’s ability to use insulin-like growth factor, or IGF-1, a hormone created primarily in the liver. Growth hormone and IGF-1 work together to promote normal ...
- Imagine living in a 4-foot body that doesn’t develop chronic diseaseson April 30, 2024 at 8:34 am
That genetic disruption blocks the body’s ability to use insulin-like growth factor, or IGF-1, a hormone created primarily in the liver. Growth hormone and IGF-1 work together to promote normal ...
- Genetic Variants May Amplify Parkinson’s Risks — But How?on April 25, 2024 at 10:22 pm
Lysosomal Genes Contribute to Parkinson’s Disease near Agriculture with High-Intensity Pesticide Use - (https://www.nature.com/articles/s41531-024-00703-4) ...
via Bing News