Other decellularized plants could provide the framework for a wide range of tissue engineering technologies. “We have a lot more work to do, but so far this is very promising,” said Glenn Gaudette, PhD, professor of biomedical engineering at WPI and corresponding author of the paper. “Adapting abundant plants that farmers have been cultivating for thousands of years for use in tissue engineering could solve a host of problems limiting the field.”
In addition to Gaudette, the WPI research team includes Tanja Dominko, PhD, DVM, associate professor of biology and biotechnology, who studies molecular mechanisms of human cell development; Pamela Weathers, PhD, professor of biology and biotechnology, a plant biologist; and Marsha Rolle, PhD, associate professor of biomedical engineering, who focuses on vasculature tissue engineering. The collaborative team also includes human stem cell and plant biology researchers at Wisconsin and Arkansas. “This project speaks to the importance of interdisciplinary research,” Gaudette said. “When you have people with different expertise coming at a problem from different perspectives, novel solutions can emerge.”
The paper’s first author is Joshua Gershlak, a graduate student in Gaudette’s lab, who helped design and conduct the experiments, and who developed an effective process for removing plant cells from spinach leaves by flowing or “perfusing” a detergent solution through the leaves’ veins. “I had done decellularization work on human hearts before,” Gershlak said, “and when I looked at the spinach leaf its stem reminded me of an aorta. So I thought, let’s perfuse right through the stem. We weren’t sure it would work, but it turned out to be pretty easy and replicable. It’s working in many other plants.”
When the plant cells are washed away what remains is a framework made primarily of cellulose, a natural substance that is not harmful to people. “Cellulose is biocompatible (and) has been used in a wide variety of regenerative medicine applications, such as cartilage tissue engineering, bone tissue engineering, and wound healing,” the authors wrote.
In addition to spinach leaves, the team successfully removed cells from parsley, Artemesia annua (sweet wormwood), and peanut hairy roots. They expect the technique will work with many plant species that could be adapted for specialized tissue regeneration studies. “The spinach leaf might be better suited for a highly vascularized tissue, like cardiac tissue, whereas the cylindrical hollow structure of the stem of Impatiens capensis (jewelweed) might better suit an arterial graft. Conversely, the vascular columns of wood might be useful in bone engineering due to their relative strength and geometries,” the authors wrote.
Using plants as the basis for tissue engineering also has economic and environmental benefits. “By exploiting the benign chemistry of plant tissue scaffolds,” they wrote, “we could address the many limitations and high costs of synthetic, complex composite materials. Plants can be easily grown using good agricultural practices and under controlled environments. By combining environmentally friendly plant tissue with perfusion-based decellularization, we have shown that there can be a sustainable solution for pre-vascularized tissue engineering scaffolds.”
At WPI, the research continues along several lines, Gaudette said, with studies to optimize the decellularization process and further characterize how various human cell types grow while they are attached to, and are potentially nourished by, plant-based scaffolds. Also, engineering a secondary vascular network for the outflow of blood and fluids from human tissue will be explored. On April 7, 2017, Gershlak will present the technology and early results as an invited speaker at the National Academy of Inventors inaugural Student Innovation Showcase in Boston, where he will detail the work for more than 200 accomplished inventors and technology commercialization leaders.
Learn more: WPI Team Grows Heart Tissue on Spinach Leaves
[osd_subscribe categories=’tissue-regeneration’ placeholder=’Email Address’ button_text=’Subscribe Now for any new posts on the topic “ARTIFICIAL SYNAPSES”‘]
Receive an email update when we add a new ARTIFICIAL SYNAPSES article.
The Latest on: Tissue regeneration
[google_news title=”” keyword=”tissue regeneration” num_posts=”10″ blurb_length=”0″ show_thumb=”left”]
via Google News
The Latest on: Tissue regeneration
- Japanese researchers test cardiac spheroids for heart regeneration in monkeyson April 26, 2024 at 9:33 pm
Regenerative heart therapies involve transplanting cardiac muscle cells into damaged areas of the heart to recover lost function.
- In a first, mice brain circuits regenerated using rat stem cellson April 25, 2024 at 8:29 am
Two independent research teams have achieved successful regeneration of mouse brain circuits by cultivating neurons from rat stem cells.
- The murky, unregulated world of anti-ageing stem cell therapyon April 24, 2024 at 10:50 am
Stem cells are the new focal point of the rich and famous with Hollywood A-listers reportedly spending tens of thousands of pounds each year on expensive therapies offered by private longevity clinics ...
- Tissue Engineering and Regeneration Market [2028]: Navigating Opportunities and Challengeson April 24, 2024 at 3:11 am
USD 14.57 billion in 2022 and is poised for substantial growth, projecting a Compound Annual Growth Rate (CAGR) of 8.35% through 2028.
- New small molecule helps scientists study regenerationon April 23, 2024 at 9:23 am
Regenerating damaged tissues or organs has been a dream of scientists for decades. Now, researchers at the FMI and Novartis Biomedical Research have discovered a new molecule that activates a protein ...
- Secrets of zebrafish heart regeneration could transform human cardiac careon April 20, 2024 at 12:30 pm
Zebrafish heart tissue regeneration is a captivating prospect for transformative advances in human cardiac care ...
- Why can zebrafish regenerate damaged heart tissue, while other fish species cannot?on April 20, 2024 at 12:07 pm
A heart attack will leave a permanent scar on a human heart, yet other animals, including zebrafish, can clear cardiac scar tissue and regrow damaged muscle as adults. Biologists sheds new light on ...
- Why zebrafish can regenerate damaged heart tissue, while other fish species cannoton April 19, 2024 at 8:16 am
A heart attack will leave a permanent scar on a human heart, yet other animals, including some fish and amphibians, can clear cardiac scar tissue and regrow damaged muscle as adults.
- $33 Million to Truly Regenerate Arthritic Jointson April 19, 2024 at 8:12 am
Duke School of Medicine, Boston Children’s Hospital, and University of California Los Angeles (UCLA) have been awarded a contract of up to $33 million from the Advanced Research Projects Agency for ...
via Bing News