Screening large “libraries” of compounds to find those with a desired biological activity is a powerful method for discovering new drugs, but requires a large, expensive and dedicated facility.
Now, scientists at the Florida campus of The Scripps Research Institute (TSRI) have devised the central component of a screening system that would be orders of magnitude smaller and cheaper.
“We’ve developed a device that can do the functional equivalent of high-throughput compound screening on an ultra-miniaturized scale,” said the study’s principal investigator Brian M. Paegel, an associate professor at TSRI.
The advance, published recently online ahead of print in Analytical Chemistry, follows a previous study from the Paegel laboratory in ACS Combinatorial Science that described the synthesis of miniaturized DNA-encoded compound libraries. The new screening device is designed to work with the new type of library.
One-Bead, One-Compound
Current high-throughput screening systems typically occupy 10,000 square feet of space or more and cost millions of dollars. They rely heavily on robotic devices that retrieve compounds from the library, place each compound into a separate small well in an “assay microplate” and measure each compound’s biological activity—for example, whether the compound inhibits a particular enzyme involved in viral replication.
Being almost entirely automated and relatively quick, such systems can rapidly screen the tens or hundreds of thousands of compounds in a typical library. But the great cost of these high-throughput screening systems limits their use to locations at pharmaceutical companies and large research institutions. The Scripps Florida campus houses one of the most active high-throughput screening facilities outside the pharmaceutical industry.
The new approach starts with the use of “one-bead-one-compound” (OBOC) libraries, in which individual compounds are chemically attached to microscopic beads. Over the past two decades, many laboratories have begun to work with OBOC libraries of one type or another, which are so quickly and cheaply prepared and are so compact that such libraries are essentially laboratory consumables.
“It is possible to generate an OBOC library of millions of compounds in a week for about $500,” said Alexander K. Price, a senior research associate in the Paegel laboratory and lead author of the new study.
LIGHTSABR
There are considerable technical challenges involved in putting bead-borne compounds through miniature screening devices. But, as they report in their new paper, Paegel and Price were able to engineer a benchtop-scale device that meets these challenges and can screen OBOC libraries.
The device is built on the microfluidics principles that also underlie inkjet printer technology. Using a “suspension hopper,” which Paegel and Price described in a 2014 Analytical Chemistry paper, the device introduces OBOC library beads into tiny liquid droplets that contain the assay of interest, such as an enzymatic activity assay. The volume of these assay droplets is about 100,000 times less than the volumes used for high-throughput screening assays.
The device then frees each compound from its bead with a photochemical reaction induced by ultraviolet (UV) light and, after an appropriate period of incubation, records the result in each droplet.
Dubbed LIGHTSABR (Light-Induced and Graduated High-Throughput Screening After Bead Release) for its light-based cleavage of compounds from their carrier beads, the device overcomes significant technical hurdles concerning the smooth flow of droplets, the absorption of stray UV irradiation and calibration of the UV waveguide.
A key innovation is that the technique allows users to vary the UV illumination to adjust the amount of a compound cleaved from its bead—and thus adjust the dose of the compound being tested. The team successfully demonstrated this dosing function using an assay designed to find inhibitors of HIV-1 protease, a key enzyme involved in the replication of the virus that causes AIDS.
The next step for Paegel and Price is to apply the microfluidic LIGHTSABR and the laboratory’s DNA-encoded OBOC libraries. “In addition to antiviral compounds, we are also pursuing new antibiotics and other drug classes that address the emergence of resistance in rapidly evolving pathogens,” said Paegel.
“Hundreds of laboratories around the world could operate their own miniaturized screening facilities, using their own assays to go after targets that are of most interest to them,” said Price.
Learn more: Scripps Florida Researchers Develop ‘LIGHTSABR’—A Cheap, Portable Drug-Discovery System
The Latest on: Portable Drug-Discovery System
[google_news title=”” keyword=”Portable Drug-Discovery System” num_posts=”10″ blurb_length=”0″ show_thumb=”left”]
via Google News
The Latest on: Portable Drug-Discovery System
- Google DeepMind Unveils Next-Gen AI Drug Discovery Modelon May 9, 2024 at 6:08 am
The third version of the "AlphaFold" AI model will help scientists target diseases and design new medications.
- Google's New AI Predicts Molecular Behavior, Which Could Supercharge Drug Discoveryon May 8, 2024 at 2:50 pm
Google purchased DeepMind in 2014 for an estimated $400 million to $650 million to expand its AI capabilities. DeepMind made headlines for creating systems that could beat the world's best Go , chess ...
- Emerging Legal Terrain: IP Risks from AI's Role In Drug Discoveryon May 8, 2024 at 12:52 pm
This article explores the benefits and risks of AI-driven drug discovery from the legal perspective. Since the law governing IP rights in AI-driven drug discovery is still in its infant state, any ...
- DeepMind CEO Targets $100 Billion-Plus AI Drug Discovery Business With AlphaFoldon May 8, 2024 at 7:59 am
First AI-designed drugs may be ready for testing in “next couple of years,” Google’s AI boss Demis Hassabis tells Bloomberg Television ...
- Drug Discovery News and Researchon May 6, 2024 at 4:59 pm
Scientists at UC San Diego have developed a machine learning algorithm to simulate the time-consuming chemistry involved in the earliest phases of drug discovery, which could significantly ...
- Nuclera eProtein Discovery system installed at leading academic instituteson May 1, 2024 at 5:00 pm
The eProtein Discovery platform overcomes this unmet need by providing rapid access to challenging proteins in a benchtop system in less than 48 hours. Accelerating drug discovery processes by up ...
- Drug Discovery Program Management Softwareon March 31, 2024 at 5:00 pm
Pipeline, a drug discovery program management software platform has been launched by Aspen Biosciences to address the coordination of high-volume data management required in drug discovery endeavors.
- Drug Discoveryon March 14, 2024 at 7:18 am
The Drug Discovery programme is Alzheimer's Society's innovative approach to dementia treatment development. The result of which means new, better treatments could be available in half the time of a ...
- Polypharmacology: Drug Discovery for the Futureon February 26, 2024 at 8:11 am
Computational approaches for polypharmacology modeling will witness rapid growth and wide application in drug discovery. There exists various similarity-based, network-based and structure-based ...
- INTEGRA Introduces Its Portable 96 Channel Pipette at the Most Affordable Priceon October 8, 2023 at 1:44 pm
This ultra-compact and portable system is the most affordable electronic 96 channel pipette on the market and available in four liquid volume ranges – 0.5-12.5 µl, 5-125 µl, 10-300 µl and 50-1250 µl – ...
via Bing News