
via University of Pennsylvania
In experiments using saliva samples from COVID-19 patients, the gum, which contains the ACE2 protein, neutralized the virus, according to research led by University of Pennsylvania scientists.
A chewing gum laced with a plant-grown protein serves as a “trap” for the SARS-CoV-2 virus, reducing viral load in saliva and potentially tamping down transmission, according to a new study.
The work, led by Henry Daniell at Penn’s School of Dental Medicine and performed in collaboration with scientists at the Perelman School of Medicine and School of Veterinary Medicine, as well as at The Wistar Institute and Fraunhofer USA, could lead to a low-cost tool in the arsenal against the COVID-19 pandemic. Their study was published in the journal Molecular Therapy.
“SARS-CoV-2 replicates in the salivary glands, and we know that when someone who is infected sneezes, coughs, or speaks some of that virus can be expelled and reach others,” says Daniell. “This gum offers an opportunity to neutralize the virus in the saliva, giving us a simple way to possibly cut down on a source of disease transmission.”
Vaccinations for COVID-19 have helped change the course of the pandemic but haven’t stamped out transmission. Even people who are fully vaccinated can still become infected with SARS-CoV-2 and, according to recent research, can carry a viral load similar to those who are unvaccinated.
Prior to the pandemic, Daniell had been studying the angiotensin-converting enzyme 2 (ACE2) protein in the context of treating hypertension. His lab had grown this protein, as well as many others that may have therapeutic potential, using a patented plant-based production system. By bombarding plant material with the DNA of target proteins, they coax plant chloroplasts to take up the DNA and begin growing the proteins. The plant material, freeze-dried and ground-up, could be used as a means of delivering the protein. This system has the potential to avoid the usual obstacles to protein drug synthesis: namely, an expensive production and purification process.
Daniell’s past work on ACE2 proved fortuitous in the context of the COVID-19 pandemic. The receptor for ACE2 on human cells also happens to bind the SARS-CoV-2 spike protein. Other research groups have shown that injections of ACE2 can reduce viral load in people with severe infections.
Meanwhile, another line of work by Daniell and Penn Dental Medicine colleague Hyun (Michel) Koo has involved research to develop a chewing gum infused with plant-grown proteins to disrupt dental plaque. Pairing his insights about ACE2 with this technology, Daniell wondered if such a gum, infused with plant-grown ACE2 proteins, could neutralize SARS-CoV-2 in the oral cavity.
To find out, he reached out to Ronald Collman at Penn Medicine, a virologist and pulmonary and critical care doctor whose team, since the early stages of the pandemic, had been collecting blood, nasal swabs, saliva, and other biospecimens from COVID patients for scientific research.
“Henry contacted me and asked if we had samples to test his approach, what kind of samples would be appropriate to test, and whether we could internally validate the level of SARS-CoV-2 virus in the saliva samples,” Collman says. “That led to a cross-school collaboration building on our microbiome studies.”
To test the chewing gum, the team grew ACE2 in plants, paired with another compound that enables the protein to cross mucosal barriers and facilitates binding, and incorporated the resulting plant material into cinnamon-flavored gum tablets. Incubating samples obtained from nasopharyngeal swabs from COVID-positive patients with the gum, they showed that the ACE2 present could neutralize SARS-CoV-2 viruses.
Those initial investigations were followed by others at The Wistar Institute and Penn Vet, in which viruses, less-pathogenic than SARS-CoV-2, were modified to express the SARS-CoV-2 spike protein. The scientists observed that the gum largely prevented the viruses or viral particles from entering cells, either by blocking the ACE2 receptor on the cells or by binding directly to the spike protein.
Finally, the team exposed saliva samples from COVID-19 patients to the ACE2 gum and found that levels of viral RNA fell so dramatically to be almost undetectable.
The research team is currently working toward obtaining permission to conduct a clinical trial to evaluate whether the approach is safe and effective when tested in people infected with SARS-CoV-2 .
“Henry’s approach of making the proteins in plants and using them orally is inexpensive, hopefully scalable; it really is clever,” Collman says.
Though the research is still in early stages of development, if the clinical trials prove the gum is safe and effective, it could be given to patients whose infection status is unknown or even for a dental check-ups when masks must be removed, to reduce the likelihood of passing the virus to caregivers.
“We are already using masks and other physical barriers to reduce the chance of transmission,” says Daniell. “This gum could be used as an additional tool in that fight.”
Original Article: A chewing gum that could reduce SARS-CoV-2 transmission
More from: University of Pennsylvania | Perelman School of Medicine at the University of Pennsylvania | University of Pennsylvania School of Veterinary Medicine | Wistar Institute | Fraunhofer Society
The Latest Updates from Bing News & Google News
Go deeper with Bing News on:
SARS-CoV-2 transmission
- Study finds that SARS-CoV-2 can shut down mitochondria, the cellular “power plants”
The study authors conjecture that ongoing inhibition of the mitochondria after infection might also play a role in the symptoms of Long COVID, especially general fatigue and malaise.
- The transmission dynamics of SARS-CoV-2 among healthcare workers
In a recent study in Nature, researchers investigate the transmission of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in hospitals and its impact on the dynamics of the ...
- Environmental and behavioral factors associated with household transmission of SARS-CoV-2 in children and adolescents
were contacted for analysis of SARS-CoV-2 transmission to children and adolescents (target population of this study). Thus, the study had as its starting point adult essential workers with a diagnosis ...
- Emerging SARS-COV-2 Variants: Genomic Variations, Transmission, Pathogenesis, Clinical Impact and Interventions
Since early 2021, multiple SARS-CoV-2 variants have emerged in various countries across the globe. Of these, ‘Variant of Concern’ has been reported to be highly transmissible, infectious and escape ...
- Study sheds light on SARS-CoV-2 transmission in homes with kids
Looking back, closing schools was probably an effective way of cutting SARS-CoV-2 transmission prior to the widespread availability of safe and effective vaccines, Johnson said. That’s a key piece of ...
Go deeper with Google Headlines on:
SARS-CoV-2 transmission
[google_news title=”” keyword=”SARS-CoV-2 transmission” num_posts=”5″ blurb_length=”0″ show_thumb=”left”]
Go deeper with Bing News on:
Reducing SARS-CoV-2 transmission
- Bacterial nasopharyngeal colonisation in children in South Africa before and during the COVID-19 pandemic: an observational study
There were variable effects on the colonisation prevalence and density of bacterial organisms during the COVID-19 compared with the pre-COVID-19 period. The lower prevalence of PCV13 serotype together ...
- Study finds that SARS-CoV-2 can shut down mitochondria, the cellular “power plants”
The study authors conjecture that ongoing inhibition of the mitochondria after infection might also play a role in the symptoms of Long COVID, especially general fatigue and malaise.
- Persistence of SARS-CoV-2 antibodies over 18 months following infection: UK Biobank COVID-19 Serology Study
Since the emergence of SARS-CoV-2 in December 2019, there have been over 650 million confirmed cases of COVID-19 worldwide.1 Although vaccination is currently effective at reducing the risk of severe ...
- Scientists developing chewing gum that could reduce SARS-CoV-2 transmission
New York, Dec 4 (IANS) A chewing gum laced with a plant-grown protein serves as a "trap" for the SARS-CoV-2 virus, reducing viral load in saliva and potentially tamping down transmission, finds a new ...
- The transmission dynamics of SARS-CoV-2 among healthcare workers
In a recent study in Nature, researchers investigate the transmission of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in hospitals and its impact on the dynamics of the ...
Go deeper with Google Headlines on:
Reducing SARS-CoV-2 transmission
[google_news title=”” keyword=”reducing SARS-CoV-2 transmission” num_posts=”5″ blurb_length=”0″ show_thumb=”left”]