Long, thin, well-defined muscle fibers (top left) are in shambles after prolonged inflammation (top right), but maintain their structure (bottom left) and strength (bottom right) when exercised during the inflammation.
Exercising lab-grown human muscle autonomously blocks the damaging effects of interferon gamma
Biomedical engineers at Duke University have demonstrated that human muscle has an innate ability to ward off the damaging effects of chronic inflammation when exercised. The discovery was made possible through the use of lab-grown, engineered human muscle, demonstrating the potential power of the first-of-its-kind platform in such research endeavors.
The results appear online on January 22 in the journal Science Advances.
“Lots of processes are taking place throughout the human body during exercise, and it is difficult to tease apart which systems and cells are doing what inside an active person,” said Nenad Bursac, professor of biomedical engineering at Duke. “Our engineered muscle platform is modular, meaning we can mix and match various types of cells and tissue components if we want to. But in this case, we discovered that the muscle cells were capable of taking anti-inflammatory actions all on their own.”
Inflammation is not inherently good or bad. When the body is injured, an initial low-level inflammation response clears away debris and helps tissue rebuild. Other times, the immune system overreacts and creates an inflammatory response that causes damage, like the often deadly cytokine storms brought on by some cases of COVID-19. And then, there are diseases that lead to chronic inflammation, such as rheumatoid arthritis and sarcopenia, which can cause muscle to waste away and weaken its ability to contract.
Among many molecules that can cause inflammation, one pro-inflammatory molecule in particular, interferon gamma, has been associated with various types of muscle wasting and dysfunction. While previous research in humans and animals has shown that exercise can help mitigate the effects of inflammation in general, it has been difficult to distinguish what role the muscle cells themselves might play, let alone how they interact with specific offending molecules, such as interferon gamma.
“We know that chronic inflammatory diseases induce muscle atrophy, but we wanted to see if the same thing would happen to our engineered human muscles grown in a Petri dish,” said Zhaowei Chen, a postdoctoral researcher in Bursac’s laboratory and first author of the paper. “Not only did we confirm that interferon gamma primarily works through a specific signaling pathway, we showed that exercising muscle cells can directly counter this pro-inflammatory signaling independent of the presence of other cell types or tissues.”
To prove that muscle alone is capable of blocking interferon gamma’s destructive powers, Bursac and Chen turned to an engineered muscle platform that the laboratory has been developing for nearly a decade. They were first to grow contracting, functional human skeletal muscle in a Petri dish, and since then the lab has been improving its processes by, for example, adding immune cells and reservoirs of stem cells to the recipe.
In the current study, the researchers took these fully functional, lab-grown muscles and inundated them with relatively high levels of interferon gamma for seven days to mimic the effects of a long-lasting chronic inflammation. As expected, the muscle got smaller and lost much of its strength.
The researchers then applied interferon gamma again, but this time also put the muscle through a simulated exercise regime by stimulating it with a pair of electrodes. While they expected the procedure to induce some muscle growth, as shown in their previous studies, they were surprised to discover that it almost completely prevented the effects of the chronic inflammation. They then showed that simulated exercise inhibited a specific molecular pathway in muscle cells, and that two drugs used to treat rheumatoid arthritis, tofacitinib and baricitinib, which block the same pathway, had the same anti-inflammatory effect.
“When exercising, the muscle cells themselves were directly opposing the pro-inflammatory signal induced by interferon gamma, which we did not expect to happen,” said Bursac. “These results show just how valuable lab-grown human muscles might be in discovering new mechanisms of disease and potential treatments. There are notions out there that optimal levels and regimes of exercise could fight chronic inflammation while not overstressing the cells. Maybe with our engineered muscle, we can help find out if such notions are true.”
The Latest Updates from Bing News & Google News
Go deeper with Bing News on:
- The Best Juice to Sip on To Reduce Inflammation, Says Dietitian
This unexpected juice is highly recommended for sports recovery and reducing inflammation in the body. The post The Best Juice to Sip on To Reduce Inflammation, Says Dietitian appeared first on The ...
- COVID Vaccine Brings Distinct Adverse Events to People With Chronic Inflammatory Disease
Study offers much-needed safety data; sheds light on link between reactogenicity and immunogenicity following vaccination ...
- Nanotechnology Could Have a Crucial Role in Inflammatory Bowel Disease Treatment
A new review focuses on recent advancements in nanotechnology-based treatments for IBD, mainly targeting inflammation and dysregulated gut microbiomes.
- Prostate Cancer Recurrence After Surgery Linked to Pelvic Inflammation
A recent study is the first to examine the association between pelvic inflammation and prostate cancer biology and outcomes.
- Hydrogen generated by Si-based agent attenuates inflammation in ulcerative colitis mouse model
Many people worldwide suffer from a chronic type of inflammatory bowel disease (IBD) called ulcerative colitis (UC), which is characterized by erosions and ulcers within the mucous membrane of the ...
Go deeper with Google Headlines on:
Go deeper with Bing News on:
- Muscle Confusion Can Help You Out Of Your Fitness Rut
In essence, muscle confusion is all about keeping your body guessing. “It’s the idea that constantly changing up your workout routine every week — or even every day — 'confuses' your muscles to avoid ...
- Master the Eccentric Muscle-up for Gymnastic Strength and Upper-body Growth
Hone your form and build strength you can use with the eccentric muscle-up – a key step on your journey to gymnastics mastery ...
- I want to lose fat and build muscle at the same time. Are my light weights enough?
Light weights won't provide enough stimulus for muscle growth, so it's key to lift heavy if you want to build muscle, a personal trainer said.
- HEALTH AND FITNESS: Recovering from vacation
After two years of staying close to home you may have taken a vacation this summer. Maybe you had an active summer and maintained your fitness. More likely, you took ...
- The Leg Day Workout That’s Missing from Your Training Program
Do you really need a dedicated leg day workout? Covering all that ground should be sufficient, right? “Wrong,”, C.S.C.S., founder of WORK Training Studio in Irvine, California, tells Runner’s World.