
Jason S. McLellan, associate professor of molecular biosciences, left, and graduate student Daniel Wrapp, right, work in the McLellan Lab at The University of Texas at Austin Monday Feb. 17, 2020.
Responding to a need to quickly develop billions of doses of lifesaving COVID-19 vaccines, a scientific team at The University of Texas at Austin has successfully redesigned a key protein from the coronavirus, and the modification could enable much faster and more stable production of vaccines worldwide.
The new findings are described in the journal Science.
Most coronavirus vaccine candidates train the human immune system to recognize a key protein on the surface of the SARS-CoV-2 virus called the spike protein in order to fight infection. Researchers at University of Texas at Austin designed a new version of this protein that, when expressed in cells, produces up to 10 times more protein than that of an earlier synthetic spike protein already in use in multiple COVID-19 vaccines. Along with colleagues at the National Institutes of Health, several members of the UT research team also designed the earlier version of the spike protein found in at least two COVID-19 vaccine candidates currently in U.S. clinical trials.
“Depending on the type of vaccine, this improved version of the protein could reduce the size of each dose or speed up vaccine production,” said Jason McLellan, an associate professor in the Department of Molecular Biosciences and senior author of the paper. “Either way, it could mean more patients have access to vaccines faster.”
Dubbed HexaPro, the new protein is also more stable than the team’s earlier version of the spike protein, which should make it easier to store and transport. It also keeps its shape even under heat stress, during storage at room temperature and through multiple freeze-thaws. Such qualities are desirable in a robust vaccine.
The Bill & Melinda Gates Foundation has contributed to the development of the technology through a grant in the interest of making vaccines accessible to people in lower-income countries. Vaccine companies with different platform technologies will have the ability to test and further develop COVID vaccines that use HexaPro. McLellan has also indicated there is interest from partners in extending access to the technology to people in the developing world.
“Four billion people living in developing countries will need access to a vaccine, as all of us will,” McLellan said.
HexaPro also could be used in COVID-19 antibody tests where it would act as a probe to identify the presence of antibodies in a patient’s blood, indicating whether a person has previously been infected with the virus.
The paper’s first author is Ching-Lin Hsieh, a postdoctoral researcher in McLellan’s lab. Corresponding authors are McLellan; Ilya Finkelstein, an associate professor in the Department of Molecular Biosciences; and Jennifer Maynard, a professor in the Cockrell School of Engineering.
The team’s original version of the spike protein forms the basis of vaccine candidates currently in human clinical trials, including Moderna’s mRNA-1273 and Novavax’s NVX-CoV2373.
For nucleic acid-based vaccines that use the patient’s own cells to create the viral proteins that trigger an immune response, such as mRNA-1273, this improved spike protein might enable next-generation versions that require a much smaller dose to elicit the same immune response from a patient. For subunit vaccines that contain a version of the actual viral protein as an antigen, such as NVX-CoV2373, many more vaccine doses could be produced in the same time frame. Either way, from a production standpoint, this could mean accelerating access to lifesaving vaccines.
Drawing on their experience creating stabilized proteins as vaccines against MERS-CoV, the coronavirus that causes Middle East respiratory syndrome, and other viruses, the researchers identified 100 different modifications to the spike protein that they believed might lead to a more stable, more highly expressed version. Next they created 100 different versions of the protein by inserting the genetic blueprints for each version into a different culture of human cells. Of those 100 versions of the spike protein, 26 were more stable or had higher expression.
The researchers then took four of those beneficial modifications, plus two from their original stabilized spike protein, and combined them to create HexaPro. When they inserted the genetic blueprints for this version of the spike protein into a human cell culture, the cells produced 10 times as much protein than that of their original protein.
The Latest Updates from Bing News & Google News
Go deeper with Bing News on:
Covid-19 vaccine production
- Oil and gas sector warns emissions cap could lead to production curtailments
CALGARY - Canada's oil and gas industry says the emissions cap framework announced by Ottawa could result in significant production curtailments by ...
- COVID-19: Brazil steps up vaccination, monitors new variants
Following the identification of two subvariants of COVID-19 in Brazil (JN.1 and JG.3), the Ministry of Health announced it is monitoring the situation and has reinforced vaccination as the main means ...
- SAG-AFTRA Hit With Over 100 Covid Vaccine Mandate Suits By Members; “Claims Are Without Merit,” Guild Says
Hollywood’s vaccine mandates are gone, but as new legal actions filed today against SAG-AFTRA make clear, the battle over the Covid-19 protection is far from ... SAG-AFTRA was allowing its signatories ...
- He was fired after refusing COVID-19 vaccine. Now former Columbia sports radio host is suing
A former Columbia sports radio host is suing his former employer, claiming his civil rights were violated when he was fired in October 2021 for refusing to be vaccinated for COVID-19, according to the ...
- The Updated COVID-19 Shot Works on the Newest Variants
Every COVID-19 vaccine is a step behind the virus. In the time it takes companies to make the shot, SARS-CoV-2 is already busy mutating into different versions that can evade the immune response ...
Go deeper with Google Headlines on:
Covid-19 vaccine production
[google_news title=”” keyword=”Covid-19 vaccine production” num_posts=”5″ blurb_length=”0″ show_thumb=”left”]
Go deeper with Bing News on:
HexaPro
- Modulating SARS-CoV-2 Spike Protein Reactivity through Moderate Electric Fields: A Pathway to Innovative Therapies
Creative Commons (CC): This is a Creative Commons license. Attribution (BY): Credit must be given to the creator. Article Views are the COUNTER-compliant sum of full text article downloads since ...
- Unibet | Poker Advent Calendar
Every day throughout December, Unibet Poker will have a new Christmas mission. Complete the daily mission to earn yourself poker prizes like Bonus Points, HexaPro tickets, and even chances at scoring ...
- Unibet | Poker Halloween Missions
Your missions are as follows: Reach 31 Flops at Cash Games on 3 separate days between the 24.10.23 - 30.10.2023. Play 10 HexaPro games on 3 separate days between the 24.10.23 - 30.10.2023. The €3,000 ...
- Unibet Handing Out €250,000 to Kick Off 2023
The €65,000 XP Point Races are for everybody. Five leaderboard spread over five weeks where players earn points from cash games, tournaments, and the speedy HexaPro games. All you need to do is play ...
Go deeper with Google Headlines on:
HexaPro
[google_news title=”” keyword=”HexaPro” num_posts=”5″ blurb_length=”0″ show_thumb=”left”]