Visually indistinguishable particles of Brome Mosaic Virus. (Ayala Rao/UCR)
UC Riverside scientists have solved a 20-year-old genetics puzzle that could result in ways to protect wheat, barley, and other crops from a devastating infection.
Ayala Rao, professor of plant pathology and microbiology, has been studying Brome Mosaic virus for decades. Unlike some viruses, the genetic material of this virus is divided into three particles that until now were impossible to tell apart.
“Without a more definitive picture of the differences between these particles, we couldn’t fully understand how they work together to initiate an infection that destroys food crops,” Rao said. “Our approach to this problem has brought an important part of this picture into very clear focus.”
A paper describing the work Rao’s team did to differentiate these particles was recently published in the Proceedings of the National Academy of Sciences.
Inside each of the particles is a strand of RNA, the genetic material that controls the production of proteins. The proteins perform different tasks, some of which cause stunted growth, lesions, and ultimately death of infected host plants.
Two decades ago, scientists used the average of all three particles to create a basic description of their structure. In order to differentiate them, Rao first needed to separate them, and get them into their most pure form.
Using a genetic engineering technique, Rao’s team disabled the pathogenic aspects of the virus and infused the viral genes with a host plant.
“This bacterium inserts its genome into the plant’s cells, similar to the way HIV inserts itself into human cells,” Rao said. “We were then able to isolate the viral particles in the plants and determine their structure using electron microscopes and computer-based technology.”
Now that one of the particles is fully mapped, it’s clear the first two particles are more stable than the third.
“Once we alter the stability, we can manipulate how RNA gets released into the plants,” Rao said. “We can make the third particle more stable, so it doesn’t release RNA and the infection gets delayed.”
This work was made possible by a grant from the University of California Multicampus Research Program and Initiatives. Professors Wiliam Gelbart, Chuck Knobler, and Hong Zhou of UCLA, as well as graduate students Antara Chakravarthy of UCR and Christian Beren of UCLA, made significant contributions to this project.
Moving forward, Rao is hoping to bring the other two viral particles into sharper focus with the expertise of scientists at UCLA and UC San Diego.
Brome Mosaic virus primarily affects grasses such as wheat and barley, and occasionally affects soybeans as well. According to Rao, it is nearly identical to Cucumber Mosaic virus, which infects cucumbers as well as tomatoes and other crops that are important to California agriculture.
Not only could this research lead to the protection of multiple kinds of crops, it could advance the understanding of any virus.
“It is much easier to work with plant viruses because they’re easier and less expensive to grow and isolate,” Rao said. “But what we learn about the principles of replication are applicable to human and animal viruses too.”
The Latest Updates from Bing News & Google News
Go deeper with Bing News on:
Brome Mosaic virus
- New winter wheat variety features broad adaptation, high yield potentialon August 23, 2021 at 11:04 am
A new hard red winter wheat variety introduced on a limited basis in 2020 will be widely available as certified seed for the 2021 winter wheat planting season.
Go deeper with Google Headlines on:
Brome Mosaic virus
Go deeper with Bing News on:
- Dallas: Where A Spray Today May Keep West Nile Virus At Bayon August 24, 2021 at 5:03 pm
The city is battling those resilient mosquitos in hopes of eradicating the West Nile virus they carry. See how you can help.
- Scientists research plant-based COVID vaccine in West Palm Beachon August 24, 2021 at 2:32 pm
Palm Beach Research Center is currently recruiting participants for a plant-based COVID-19 vaccine study. Kentucky Bio Processing, based in Owensboro, Kentucky, is growing tobacco plants for a ...
- Funding boosted for early detection of zoonotic diseaseson August 24, 2021 at 12:47 pm
APHIS receives $300M for emerging virus surveillance as part of One Health focus between human and animal diseases.
- USDA Announces Proposed Framework for Advancing Surveillance for SARS-CoV-2 and Other Emerging Zoonotic Diseases through the American Rescue Planon August 24, 2021 at 9:46 am
The U.S. Department of Agriculture is dedicating $300 million in American Rescue Plan Act funding to conduct surveillance for SARS-CoV-2 and other emerging and zoonotic diseases in susceptible animals ...
- 2 Mystery Plant Viruses a New Worry for Watermelon Growers in Floridaon August 24, 2021 at 6:17 am
It appears crinkle leaf-associated virus 1 and 2 have made it to the Sunshine State. But there is still much to learn about the pathogens.