Engineering researchers developed a next-generation miniature lab device that uses magnetic nano-beads to isolate minute bacterial particles that cause diseases. Using this new technology improves how clinicians isolate drug-resistant strains of bacterial infections and difficult-to-detect micro-particles such as those making up Ebola and coronaviruses.
Ke Du and Blanca Lapizco-Encinas, both faculty-researchers in Rochester Institute of Technology’s Kate Gleason College of Engineering, worked with an international team to collaborate on the design of the new system—a microfluidic device, essentially a lab-on-a-chip.
Drug-resistant bacterial infections are causing hundreds of thousands of deaths around the world every year, and this number is continuously increasing. Based on a report from the United Nations, the deaths caused by antibiotics resistance could reach to 10 million annually by 2050, Du explained.
“It is urgent for us to better detect, understand, and treat these diseases. To provide rapid and accurate detection, the sample purification and preparation is critical and essential, that is what we are trying to contribute. We are proposing to use this novel device for virus isolation and detection such as the coronavirus and Ebola,” said Du, an assistant professor of mechanical engineering whose background is in development of novel biosensors and gene editing technology.
The lab team is interested in the detection of bacterial infection, especially in bodily fluids. One of the major problems for detection is how to better isolate higher concentrations of pathogens.
The device is a sophisticated lab environment that can be used in field hospitals or clinics and should be much faster at collecting and analyzing specimens than the commercially available membrane filters. Its wide, shallow channels trap small bacteria molecules that are attracted to packed, magnetic microparticles.
This combination of the deeper channels on the nano-device, increased flow rate of fluids where bacteria are suspended, and the inclusion of magnetic beads along the device channels improves upon the process of capturing/isolating bacterial samples. Researchers were able to successfully isolate bacteria from various fluids with a microparticle-based matrix filter. The filter trapped particles in small voids in the device, providing a larger concentration of bacteria for analysis. An added advantage of a smaller device such as this allows for multiple samples to be tested at the same time.
“We can bring this portable device to a lake which has been contaminated by E. coli. We will be able to take a few milliliters of the water sample and run it through our device so the bacteria can be trapped and concentrated. We can either quickly detect these bacteria in the device or release them into certain chemicals to analyze them,” said Du, whose earlier work focused on devices that use the CRISPR gene-editing technology and the fundamental understanding of fluidic dynamics.
Teaming up with Lapizco-Encinas, a biomedical engineer with expertise in dielectrophoresis—a process that uses electrical current to separate biomolecules—their collaboration provided the increased capability toward better pathogen detection, specifically for bacteria and microalgae isolation and concentration.
“Our goal is not only isolating and detecting bacteria in water and human plasma, but also working with whole blood samples to understand and detect blood infection such as sepsis. We already have a concrete plan for that. The idea is to use a pair of the nano-sieve devices for sequential isolation,” said Lapizco-Encinas, an associate professor in RIT’s biomedical engineering department.
The Latest Updates from Bing News & Google News
Go deeper with Bing News on:
- DOH says no Nipah virus case detected in PH, urges masking amid reported rise in flu cases in CDO
The Department of Health Northern Mindanao has denied the country has detected cases of the feared Nipah virus after a reported rise in fever and flu-like illnesses in schools and several barangays in ...
- Appdome releases new defenses to combat accessibility malware
Two of the most advanced variants focus on Android banking apps - BrasDex in Latin America and Xenomorph in the U.S. and Europe use Automated Transfer Systems (ATS) malware.
- Additional West Nile virus cases confirmed in 4 Washington counties
Two people and two horses in Washington state have had confirmed cases of West Nile virus diagnosed in the past few days.
- Killer T cells in older adults resemble those found in newborns, but fall short in virus detection
A world-first discovery has revealed special immune cells called 'killer T cells' in older adults, directed against influenza viruses, closely resemble those found in newborns and children, but ...
- Study finds immune cells in older adults resemble those in newborns and children, but fall short in virus detection
A world-first discovery has revealed special immune cells called "killer T cells" in older adults, directed against influenza viruses, closely resemble those found in newborns and children, but ...
Go deeper with Google Headlines on:
[google_news title=”” keyword=”virus detection” num_posts=”5″ blurb_length=”0″ show_thumb=”left”]
Go deeper with Bing News on:
- New technology could pave the way to rapid infection testing
New technology could pave the way to a future of rapid testing in hospitals and at home for both covid and urinary tract infection.
- Bacterial biosensors: The future of analyte detection
Scientists can do some nifty things with microbes, including engineering bacterial cells to sense and signal the presence of specific compounds. These microbial whole-cell biosensors have numerous ...
- E. coli detection prompted temporary boil order in Bondsville
The Bondsville Water District lifted a boil water order on Sept. 20, four days after E. coli was found in water samples collected Sept. 15. A second sample tested for Coliform, which indicated fecal ...
- Glow-in-the-dark bacteria could detect millions of landmines
Gideon Lapidoth was clearing mines in the Golan Heights. It was night and his protective goggles had fogged up — that was when he felt his foo ...
- New Technic for Detection of Bacterial Contamination in a Blood Bank Using Plastic Equipment
air embolism or homologous serum jaundice — bacterial contamination is the most insidious and dangerous. Contamination is difficult to detect because the very procedure of sampling a container ...
Go deeper with Google Headlines on:
[google_news title=”” keyword=”bacteria detection” num_posts=”5″ blurb_length=”0″ show_thumb=”left”]