A new Trojan horse approach could lead to treatments for some antibiotic-resistant bacteria.
A deadly, antibiotic-resistant bacterium can be sterilized by hijacking its haem-acquisition system, which is essential for its survival. The new strategy, developed by Nagoya University researchers and colleagues in Japan, was published in the journal ACS Chemical Biology.
Pseudomonas aeruginosa is a dangerous bacterium that causes infections in hospital settings and in people with weakened immune systems. It can cause blood infections and pneumonia, while severe infections can be deadly. Highly resistant to antibiotic treatment, P. aeruginosa is one of the most critical pathogens urgently requiring alternative treatment strategies, according to the World Health Organization.
This bacterium is one of many that have evolved a system that allows them to acquire difficult-to-access iron from the human body. Iron is essential for bacterial growth and survival, but in humans, most of it is held up within the ‘haem’ complex of haemoglobin. To get hold of it, P. aeruginosa and other bacteria secrete a protein, called HasA, which latches onto haem in the blood. This complex is recognized by a membrane receptor on the bacterium called HasR, permitting haem entry into the bacterial cell, while HasA is recycled to pick up more haem.
Bioinorganic chemist Osami Shoji of Nagoya University and collaborators have found a way to hijack this ‘haem acquisition system’ for drug delivery. They developed a powder formed of HasA and the pigment gallium phthalocyanine (GaPc), which, when applied to a culture of P. aeruginosa, was consumed by the bacteria.
“When the pigment is exposed to near-infrared light, harmful reactive oxygen species are generated inside the bacterial cells,” explains Shoji. When tested, over 99.99% of the bacteria were killed following treatment with one micromolar of HasA with GaPc and ten minutes of irradiation.
The strategy also worked on other bacteria with the HasR receptor on their membranes, but not on ones without it.
The haem acquisition system is so essential to these bacteria’s survival that it is not expected to change, making it unlikely the bacteria will develop resistance to this drug strategy, the researchers believe.
“Our findings support the use of artificial haem proteins as a Trojan horse to selectively deliver antimicrobials to target bacteria, enabling their specific and effective sterilization, irrespective of antibiotic resistance,” the team reports in their study.
The researchers next aim to test their strategy for treating infections, and are working on modifying their approach for sterilizing other pathogens that possess a similar haem acquisition system.
The Latest on: Antibiotic resistance
via Google News
The Latest on: Antibiotic resistance
- University of Oxford: Antimicrobial resistant bacteria found in newborn children from low- and middle-income countrieson August 6, 2022 at 4:13 am
Sepsis is a primary cause of mortality in newborns, particularly in low- and middle-income countries (LMICs). A new study coordinated by Professor Tim Walsh at the Ineos Oxford Institute for ...
- Lagos woman Reveals Infection treatment Remedy for Staph Aureus, Syphilis, Gonorrhea, e.coli, std and other Resistant Infectionson August 6, 2022 at 4:00 am
If you want to get rid of staphylococcus aureus, syphilis, std, gonorrhea and other stubborn kinds of infections once and for all ...
- Antimicrobial resistance said to pose a public-health threaton August 6, 2022 at 2:00 am
The South African health system grapples with antimicrobial resistance following a study that indicates a shortage of infectious diseases physicians practising countrywide.
- A new biosynthesis method has been developed to produce antibiotics from natural substanceson August 5, 2022 at 1:31 pm
The use of the element fluorine to modify active substances is an important tool in modern drug development. A team at Goethe University Frankfurt has now achieved an important “first” by successfully ...
- Early-life acquisition of antibiotic resistance in neonates from developing countrieson August 4, 2022 at 10:01 pm
Every year, almost 7 million potentially serious bacterial infections are estimated to occur in newborns, resulting in more than 550,000 annual neonatal deaths.
- Antibiotic-Resistant Bacteria Emerging in Community Settingson August 4, 2022 at 11:28 am
CREs are a group of multidrug-resistant bacteria considered an urgent health threat by the CDC because they can rapidly spread between patients, especially those who are most seriously ill and ...
- Early-life acquisition of antimicrobial resistance in newborn children from low- and middle-income countrieson August 4, 2022 at 8:00 am
Every year, almost 7 million potentially serious bacterial infections are estimated to occur in newborns, resulting in more than 550,000 annual neonatal deaths. Most of these infections and deaths ...
- What is the prevalence of carbapenem-resistant Enterobacterales among individuals with and without health risk factors?on August 3, 2022 at 6:45 am
Researchers assessed carbapenem-resistant Enterobacterales (CRE) epidemiology across eight US metropolitan areas between 1 January 2012 and 31 December 2015.
- Feeding Dogs Raw Meat Might Exacerbate The Antibiotic Resistance Crisison July 31, 2022 at 1:56 pm
In a new study published in the Journal of Antimicrobial Chemotherapy, a team of researchers at the University of Bristol observed that pet dogs who were predominantly on a diet of raw meat were far ...
- From COVID-19 to antibiotic resistance, we need to tap nature to find new drugson July 27, 2022 at 8:00 pm
While humans evolved over a period of approximately 6 million years, breakthroughs in modern medicine as we know it today got going only in the 19th and 20th centuries. So how did humans successfully ...
via Bing News