Many tasks in biology require tiny, accurate motion – achieved with expensive hardware. We have used inexpensive, 3D printed parts to make high performance mechanisms for low cost science.
Our best example is a microscope small and cheap enough to be left in an incubator or fume hood for days or weeks. This will enable new science, for example by observing cells as they grow in an incubator. We will improve this microscope’s imaging capabilities (adding fluorescence and phase contrast) and demonstrate its use in an incubator. We will also show that printed mechanisms can be used for other tasks, for example the mechanical manipulation of micropipettes for microinjection or patch clamping.
Optical microscopy is fundamental to biology, and relatively high performance microscopes can now be made very cheaply. Positioning the sample and focusing the objective, however, is difficult without expensive translation stages: a microscope is mostly mechanics. Many other tasks in biology require tiny, accurate motion – achieved with expensive hardware such as mechanical micromanipulators and piezoelectric actuators. We have used inexpensive, 3D printed parts to make high performance mechanisms for low cost science, and we propose to apply this technology to problems in synthetic biology.
Our best example is a microscope small and cheap enough to be left in an incubator or fume hood for days or weeks. This will enable new science, for example by observing cells as they grow in an incubator – experiments which are currently impossible to do on a large scale due to the time and resources required. We will improve this microscope’s biological imaging capabilities (adding fluorescence and phase contrast) and demonstrate its use in an incubator at the Light Microscopy Facility in the Cancer Research Institute. This will then allow us to study phototoxicity by monitoring cultures of cells over several days.
Plastic flexure technology could also reduce the cost of mechanical micromanipulators by three orders of magnitude, opening up a range of possibilities. When combined with open-source Arduino microcontrollers, it is even possible to automate these devices for around £100. We will develop and test plastic micromanipulators for microinjection or electrophysiology, and assess their precision and stability. We will also investigate the use of ABS plastic as a potential replacement for PLA, as it has the potential to further improve the performance of printed mechanisms.
Finally, these low cost devices present obvious opportunities for science outreach, and this funding would enable us to create a class set of microscopes that can be taken (or lent) to schools as part of outreach activities, along with some fixed samples and lesson plans for easily-prepared specimens.
Learn more: Open source 3D-printed microscope
The Latest on: 3D printed microscope
via Google News
The Latest on: 3D printed microscope
- Microscopic imaging without a microscope?on June 10, 2021 at 12:35 pm
A new technique uses high-throughput sequencing, instead of a microscope, to obtain ultra-high-resolution images of gene expression from a tissue slide. The 30,000 or so genes making up the human ...
- Modified 3D Printer Makes A Great Microscope, Tooon June 9, 2021 at 5:00 pm
Look past the melty plastic bits, and your average 3D printer is just a handy 3-axis ... easily be modified into an automated polarimetric microscope! The microscope build actually took two ...
- New atomic-scale 3D map of Covid virus protein could hold clue to preventing lung damageon June 8, 2021 at 7:00 pm
Researchers in US use cryo-electron microscope to construct 3D map of virus protein that reveals how Covid-19 infects human lungs.
- (PDF) Medical Holography Market: Hologram the future of medicine | Detailed Study by Coherent Market Insights with Upcoming Trendson June 8, 2021 at 7:06 am
For instance, in September 2017, HoloTech Switzerland AG acquired 3D holographic print division from Zebra ... developed a microscope-based on holography and image reconstruction algorithm.
- Google and Harvard Unveil the Largest High-Resolution Map of the Brain Yeton June 6, 2021 at 7:00 am
Last Tuesday, teams from Google and Harvard published an intricate map of every cell and connection in a cubic millimeter of the human brain.
- 3D-Printed Laser Scanning Confocal Microscope Measures Micronson June 4, 2021 at 5:00 pm
Not all microscopes are created equal, though, with some being far more optimized for making fine measurements of the microscopic realm. This 3D-printed confocal laser scanning microscope is a ...
- 3D Printing with Injection Molding Precisionon June 3, 2021 at 6:00 am
Boston Micro Fabrication (BMF) has developed a new 3D printing process to create millimeter-sized products with details at the micron scale.
- Nanophotonics and nanofabrication combine to achieve 3D color printson June 2, 2021 at 3:55 am
"Unlike previous 3D color holograms, our color-filtering microlens ... appear to light up and disappear with the changing focus of an optical microscope." To reduce print time and increase print ...
- 3D-Printing Technique Paves Way for Fabrication of Devices Inside the Bodyon June 1, 2021 at 5:00 pm
A new laser-based 3D printing technique that uses optical fibers could be ... lateral and 21.5-micron axial printing resolution—so far only have been created on a microscope slide. However, ...
- Medical Holography Market Size Estimated to Reach $4.12 Billion by 2025on May 31, 2021 at 2:36 am
The global Medical Holography Market based on product type has Holographic display, Holographic Microscope, Holographic print ... and displays for the 3D visualization, making the learning ...
via Bing News