Scientists from the German Cancer Research Center (DKFZ) and the stem cell institute HI-STEM* in Heidelberg have succeeded for the first time in directly reprogramming human blood cells into a previously unknown type of neural stem cell.
These induced stem cells are similar to those that occur during the early embryonic development of the central nervous system. They can be modified and multiplied indefinitely in the culture dish and can represent an important basis for the development of regenerative therapies.
Stem cells are considered to be the all-rounders of our tissues: they can multiply indefinitely and then – if they are pluripotent embryonic stem cells – generate all conceivable cell types. In 2006, the Japanese scientist Shinya Yamanaka recognized that such cells could also be produced in the laboratory – from mature body cells. Four genetic factors alone are sufficient to reverse the course of development and produce so-called induced pluripotent stem cells (iPS) that have identical properties to embryonic stem cells. Yamanaka was awarded the Nobel Prize for Medicine in 2012 for this discovery.
“This was a major breakthrough for stem cell research,” said Andreas Trumpp, German Cancer Research Center (DKFZ) and Director of HI-STEM in Heidelberg. “This applies in particular to for research in Germany, where the generation of human embryonic stem cells is not permitted. Stem cells have enormous potential both for basic research and for the development of regenerative therapies that aim to restore diseased tissue in patients. However, reprogramming is also associated with problems: For example, pluripotent cells can form germ line tumors, so-called teratomas.
Another possibility is not to completely turn back the course of development. For the first time, Trumpp’s team has succeeded in reprogramming mature human cells in such a way that a defined type of induced neural stem cells is produced that can multiply almost indefinitely. “We used four genetic factors like Yamanaka, but different ones for our reprogramming,” explains Marc Christian Thier, first author of the study. “We assumed that our factors would allow reprogramming to an early stage of development of the nervous system.”
In the past, other research groups also reprogrammed connective tissue cells into mature nerve cells or neural precursor cells. However, these artificially produced nerve cells often could not be expanded and could therefore hardly be used for therapeutic purposes. “Often, it was a heterogeneous mixture of different cell types that might not exist in the body under physiological conditions,” said Andreas Trumpp explaining the problems.
Together with stem cell researcher Frank Edenhofer from the University of Innsbruck and neuroscientist Hannah Monyer from DKFZ and the Heidelberg University Hospital, Trumpp and his team have succeeded in reprogramming different human cells: connective tissue cells of the skin or pancreas as well as peripheral blood cells. “The origin of the cells had no influence on the properties of the stem cells,” said Thier. In particular, the possibility of extracting neural stem cells from the blood of patients without invasive intervention is a decisive advantage for future therapeutic approaches.
What is special about the reprogrammed cells of the Heidelberg researchers is that they are a homogeneous cell type that resembles a stage of neural stem cells that occurs during the embryonic development of the nervous system. “Corresponding cells exist in mice and probably also in humans during early embryonic brain development,” said Thier. “We have described here a new neural stem cell type in the mammalian embryo.
These so called “induced Neural Plate Border Stem Cells” (iNBSCs) have a broad development potential. The iNBSCs of the Heidelberg scientists are expandable and multipotent and can develop in two different directions. On the one hand, they can take the path of development to mature nerve cells and their supplier cells, the glial cells, i.e. become cells of the central nervous system. On the other hand, they can also develop into cells of the neural crest, from which different cell types emerge, for example peripheral sensitive nerve cells or cartilage and bones of the skull.
The iNBSCs thus form an ideal basis for generating a broad range of different cell types for an individual patient. “These cells have the same genetic material as the donor and are therefore presumably recognized as “self” by the immune system and are not rejected,” explains Thier.
The CRISPR/Cas9 gene scissors can be used to modify the iNBSC or repair genetic defects, as the scientists have shown in their experiments. “They are therefore of interesting both for basic research and the search for new active substances and for the development of regenerative therapies, for example in patients with diseases of the nervous system. However until we can use them in patients, a lot of research work will still be necessary,” emphasizes Trumpp.
The Latest on: Regenerative medicine
via Google News
The Latest on: Regenerative medicine
- Global Regenerative Medicine Market 2020 Research Report with COVID-19 Impact, by Future Trend, Growth rate and Industry Analysis to 2025on January 19, 2021 at 6:44 pm
The statistical report titled Global Regenerative Medicine Market 2020 by Company, Type and Application, Forecast to 2025 has all the important market aspects penciled down in a understandable ...
- Parent Project Muscular Dystrophy Invests $1 Million in Satellos Bioscience to Support New Regenerative Medicine Technologyon January 19, 2021 at 9:35 am
PRNewswire/ -- Parent Project Muscular Dystrophy (PPMD), a nonprofit organization leading the fight to end Duchenne muscular dystrophy (Duchenne), today announced a $1 million programmatic investment ...
- New institute to leverage experts to advance regenerative medicineon January 19, 2021 at 6:20 am
UTSA has launched a collaborative research institute that will benefit people suffering from a variety of vascular and other medical issues. The lnstitute of Regenerative Medicine will provide a ...
- IU School of Medicine Offers New Program for Innovative Health Careon January 12, 2021 at 4:22 pm
Indiana University School of Medicine launched a new PhD program to train the next generation on a new dimension of health care.
- Direct Biologics Announces New Advanced Tool in Regenerative Medicine with Launch of AmnioWrap™ Placental Allografton January 12, 2021 at 11:47 am
Direct Biologics, LLC, an industry leader in extracellular vesicle technology, announced today that the company has launched ...
- Organogenesis ReNu® Receives FDA Regenerative Medicine Advanced Therapy (RMAT) Designation For ...on January 12, 2021 at 1:43 am
RMAT Designation Enables Closer FDA Interactions to Support Accelerated Approval CANTON, Mass., Jan. 11, 2021 (GLOBE NEWSWIRE) -- Organogenesis Holdings Inc. (Nasdaq: ORGO), a leading regenerative ...
- Organogenesis ReNu® Receives FDA Regenerative Medicine Advanced Therapy (RMAT) Designation For Osteoarthritis of the Kneeon January 11, 2021 at 6:22 am
FDA Determines ReNu Preliminary Clinical Evidence Indicates Potential to Address Unmet Medical Needs for the Management of Symptoms Associated ...
- Regenerative Medicine Market to Reach Valuation US$ 23.7 Bn by 2027on January 8, 2021 at 9:55 am
The global regenerative medicine market is representing impressive CAGR of 16.1% during the forecast period 2020 to 2027. Regenerative medicine is the division of medicine that promotes methods to ...
- Envisioning Future Trends in Regenerative Medicineon January 8, 2021 at 5:03 am
Many areas of clinical medicine could be transformed by advances in regenerative medicine, a field that includes cell therapy, gene therapy, gene modified cell therapy, and tissue engineering. Over ...
- Cyrano Therapeutics: Regenerative medicine company taking smell loss treatment candidate to the clinicon January 8, 2021 at 2:00 am
Meet Cyrano Therapeutics, a regenerative medicine company working to restore the loss of taste and smell. It’s one of our Startups to Watch for 2021.
via Bing News