
via CU Boulder
A genetic disruption strategy developed by CU Boulder researchers effectively stymies the evolution of antibiotic-resistant bacteria such as E. coli, giving scientists a crucial leg up in the ongoing battle against deadly superbugs.
These multidrug-resistant pathogens—which adapt to current antibiotics faster than new ones can be created—infect nearly 2 million people and cause at least 23,000 deaths annually in the U.S., according to data from the Centers for Disease Control.
In an effort to develop a sustainable long-term solution, CU Boulder researchers created the Controlled Hindrance of Adaptation of OrganismS (CHAOS) approach, which uses CRISPR DNA editing techniques to modify multiple gene expressions within the bacteria cells, stunting the pathogen’s central processes and thwarting its ability to evolve defenses.
The findings are outlined today in the journal Communications Biology and could open new research avenues on how to best restrict a pathogen’s antibiotic resistance.
“We now have a way to cut off the evolutionary pathways of some of the nastiest bugs and potentially prevent future bugs from emerging at all,” said Peter Otoupal, lead author of the study and a doctoral researcher in CU Boulder’s Department of Chemical and Biological Engineering (CHBE).
The CHAOS research is the culmination of work that began in 2013, when Otoupal and his colleagues began searching for genes that could act as a cellular kill switch for E. coli. When the scientists tweaked one gene at a time, the bacteria could adapt and survive. But when they altered two or more genes at once, the cell got weaker.
“We saw that when we tweaked multiple gene expressions at the same time—even genes that would seemingly help the bacteria survive—the bacteria’s fitness dropped dramatically,” Otoupal said.
The CHAOS method takes advantage of this effect, pulling multiple genetic levers in order to build up stress on the bacterial cell and eventually trigger a cascading failure, leaving the bug more vulnerable to current treatments. The technique does not alter the bug’s DNA itself, only the expression of individual genes, similar to the way a coded message is rendered useless without the proper decryption.
“You can think of it in terms of a series of escalating annoyances to the cell that eventually cause it to weaken,” said Anushree Chatterjee, senior author of the study and an assistant professor in CHBE. “This method offers tremendous potential to create more effective combinatorial approaches.”
Although E. coli has nearly 4,000 individual genes, the exact gene modification sequence appears to matter less than the sheer number of genes that are disrupted, Otoupal said. Still, the researchers plan to continue optimizing the CHAOS method to seek out the most efficient disruptions.“Diseases are very dynamic, so we need to design smarter therapies that can gain control over their rapid adaptation rates,” Chatterjee said. “The emphasis in our lab is demonstrating the efficacy of these methods and then finding ways to translate the technology to modern clinical settings.”
“In the past, nobody really considered that it might be possible to slow down evolution,” Otoupal said. “But like anything else, evolution has rules and we’re starting to learn how to use them to our advantage.”
Learn more: How to stop an antibiotic-resistant superbug
The Latest on: Genetic disruption
[google_news title=”” keyword=”genetic disruption” num_posts=”10″ blurb_length=”0″ show_thumb=”left”]
via Google News
The Latest on: Genetic disruption
- HEALTH: OMRF scientists help identify cause of brain disorderon November 26, 2023 at 7:20 pm
Oklahoma Medical Research Foundation scientists — and a lot of tiny fish — were instrumental in a worldwide effort to pinpoint the cause of a rare, severe neurodevelopmental disorder that shares some ...
- Genetic Testing Insurance Coverage Trendson November 26, 2023 at 4:01 pm
Aim: Health plan coverage policies can influence utilization of genetic tests. Our goal was to characterize the current state of test-related policies from US private payers. Methods: We searched ...
- 9 unusual facts about genetics you may not already knowon November 25, 2023 at 6:47 am
Our genetic code determines everything about us, from the color of our eyes to our susceptibility to certain diseases. Here are 9 facts you may not already know about genetics. DNA serves as the ...
- How Menopause Changes the Brainon November 24, 2023 at 5:08 am
Across the U.S., roughly 6 million adults 65 and older have Alzheimer’s disease. Almost two-thirds of them are women — a discrepancy that researchers have long attributed to genetics and women’s ...
- Genetics and Lawon November 23, 2023 at 4:01 pm
Explore the implications of genetic advances for individuals and society; as well as the law and legal issues surrounding them. You'll learn about the latest advances in genetic science, their ...
- Genetic vulnerability of leukemia cells uncoveredon November 22, 2023 at 4:48 pm
Leukemia is the most common type of cancer in children. Treatment involves intensive chemotherapy, which has severe side effects due to its non-specific mode of action.
- Researchers develop new method for prenatal genetic testingon November 21, 2023 at 4:00 pm
Researchers have developed a non-invasive genetic test that can screen the blood of pregnant individuals to survey all genes from the fetal genome. A team of investigators from Massachusetts ...
- Bear genes exhibit circadian rhythms even when hibernatingon November 19, 2023 at 8:48 am
A genetic study found that grizzly bears internal clocks continue to tick during hibernation This tenacity highlights the significance of circadian cycles in ...
- Genetics Newson November 16, 2023 at 4:00 pm
The research indicates that the genetic variant observed, which affects zinc regulation, could have signified an evolutionary advantage in our ancestors’ adaptation to the cold….
via Bing News