A team of scientists from the Helmholtz Zentrum München and the Technical University of Munich (TUM) has successfully developed a new type of microscope. The so-called NeuBtracker is an open source microscope that allows to observe neuronal activities of zebrafish without perturbing their behavior. This is opening up completely new perspectives for science, because now it will be possible to track natural behavior while simultaneously imaging neuronal activity in the brain.
NeuBtracker* is equipped with two cameras: One tracks the unrestrained behavior of the zebrafish larva while the other automatically remains pointed at the transparent head, and consequently the brain, to record fluorescence images. “This approach makes it possible to observe neuronal activity during unrestrained behavior. We can test the larvae in different environmental conditions and can immediately analyze the effects,” says Prof. Dr. Gil Westmeyer from the Institutes of Biological and Medical Imaging (IBMI) and Developmental Genetics (IDG) at the Helmholtz Zentrum München as well as the Department of Nuclear Medicine and Munich School of Bioengineering (MSB) at the Technical University of Munich (TUM).
For example, by adding substances that influence metabolism, it is possible to observe resulting neuronal events in the brain in vivo. “Now we can finally simultaneously observe the effects of physiologically active substances on the behavior and brain activity,” states Panagiotis Symvoulidis from the TUM and the Helmholtz Zentrum München and the study’s first author. “The selective expression of fluorescent sensor proteins allows us to detect the activity of particular neurons.” “Consequently we can see exactly which areas in the brain are active during specific behaviors,” adds Dr. Antonella Lauri from Westmeyer’s team.
A microscope that anyone can build
The new instrument is a so-called open-source microscope. This means that instructions on how to build the microscope are available to everyone on the web (www.neubtracker.org). “We wanted to give our scientific colleagues the possibility to build their own NeuBtracker because we had been waiting for such a device for years,” Westmeyer explains. “It is finally possible to see the effects of pharmacological substances on the behavior and the neuronal activity – or other cellular signal processing events – at the same time and across an entire organism. This systemic approach enables us to make new discoveries and we will for example seek to use this device in drug discovery and metabolic research,” Westmeyer adds.
The Latest on: Open-source microscope
- 3D-Printed Laser Scanning Confocal Microscope Measures Micronson April 28, 2021 at 5:00 pm
The stage of [Zach]’s microscope is based on OpenFlexure’s Delta Stage, an open-source, 3D-printed delta-bot motion control platform that’s capable of positioning samples with sub-micron ...
- Red Hat CEO calls on partners to get behind hybrid cloudon April 28, 2021 at 1:35 am
Open source player is holding its Summit event, with the partner keynote session seeing CEO Paul Cormier promise more improvements for partners.
- Small images, big picture: Artificial intelligence to revolutionize microscopyon April 23, 2021 at 11:00 am
The microscope, a mainstay of most laboratories, gets a makeover. Enter the age of AI- and deep-learning-powered microscopes.
- Putting ‘fair information practices’ under the microscopeon April 15, 2021 at 3:16 am
For some 50 years, “fair information practices,” or FIPs, have been the watchword in privacy protection. But 50 years along, privacy is still threatened. In an AEI paper released this week, I ...
- The Worldwide Electron Microscopy and Sample Preparation Industry is Expected to Reach $3.5 Billion by 2026 at a CAGR of 9.5% from 2021on April 8, 2021 at 5:25 am
For E.S.T Office Hours Call +1-917-300-0470 For U.S./CAN Toll Free Call +1-800-526-8630 ...
- Global Electron Microscopy and Sample Preparation Market (2021 to 2026) - Increasing Application Areas of Microscopy Presents Opportunitieson April 7, 2021 at 1:52 am
CONTACT: ResearchAndMarkets.com Laura Wood, Senior Press Manager [email protected] For E.S.T Office Hours Call 1-917-300-0470 For U.S./CAN Toll Free Call 1-800-526-8630 For GMT Office ...
- Digital Microscopes And Imaging Reveal Allon April 6, 2021 at 5:00 pm
If you’ve ever attended any optical or photonic conferences, you’ve likely seen demos of digital microscopes by Dino-Lite, a brand name for Omano Microscopes and others companies like Andonstar ...
- SINGLE: Atomic-resolution structure identification of nanocrystals by graphene liquid cell EMon April 4, 2021 at 5:01 pm
Our developments are made available through the open-source software package SINGLE ... Over the past few years, the emergence of a next generation of electron microscopes, with improvement of direct ...
via Google News and Bing News