Researchers at the RIKEN Center for Developmental Biology (CDB) have successfully transplanted retinal pigment cells derived from stem cells of one monkey into the eyes of other monkeys without rejection and without the need for immunosuppressant drugs. Published in Stem Cell Reports, the study shows that this procedure is possible as long as a set of cells called the MHC are genetically matched between the host monkey and the new retinal cells.
A realistic hope of modern medicine is to replace damaged tissue with healthy cells grown in the lab. Currently, adult cells can be reprogrammed into stem cells, and then re-differentiated and grown into desired cell types. The researchers at RIKEN CDB led by Masayo Takahashi have already begun a clinical transplant trial in people with age-related macular degeneration. The team grew retinal pigment cells from induced pluripotent stem cells (iPSCs) and transplanted them into the damaged retina of a human participant. In order to avoid tissue rejection, they used autologous iPSCs—iPSCs that were created from the recipient’s own skin cells.
While this method is sound, producing autologous iPSCs is costly. Additionally, because the cells must grow at the same rate as they do during normal development, a person would have to wait more than a year before a transplant could be performed.
Notes lead author Sunao Sugita, “In order to make iPSC transplantation a practical reality, the current goal is to create banks of iPSC-derived tissues that can be transplanted into anyone as they are needed. However, immune responses and tissue rejection are big issues to overcome when transplanting tissue derived from other individuals.”
The new study tested a technique called MHC matching as a way to overcome this issue. Major histocompatibility complexes (MHCs) are a sets of cell-surface proteins found in all cells that function in the immune system. In humans, MHCs are also called human leukocyte antigens (HLAs). There are many genetic variations of MHCs, and after transplantation, if the MHCs of the transplanted cells are not recognized by the T cells of the host immune system, there is an immune response and the tissue is rejected.
To test whether MHC matching is a viable method, the team used retinal pigment cells that were grown from monkey iPSCs in the iPS cell bank at the Center for iPS Cell Research and Application, Kyoto University. They transplanted the cells into the subretinal space in monkeys with either genetically matched or non-matched MHCs.
The researchers found that these transplanted cells survived without rejection for at least 6 months in MHC-matched monkeys, without using any of the usually necessary immunosuppressant drugs. In contrast, rejection was relatively quick in the MHC-mismatched monkeys. Immunohistochemical examination showed that infiltration by inflammatory cells was only present in the transplanted grafts of MHC-mismatched monkeys. In vitro, the team saw that T cells failed to respond to the iPSC-derived retinal pigment cells if they were from an MHC-matched monkey.
In a separate study published in the same issue of Stem Cell Reports, the researchers saw similar results when they repeated this last experiment with human T cells and HLA-matched or unmatched retinal pigment cells grown from IPSCs.
Now that we have established the lack of immune response in monkeys and in human cells in vitro,” explains Sugita, “using the iPS cell bank appears to be a viable solution, at least in the case of retinal pigment epithelial cell transplantation.”
“In the next clinical trial,” continues Sugita, “we plan to use allogeneic iPS-retinal pigment epithelial cells from HLA homozygote donors. The clinical data after the transplantation will allow us to see if the iPS cell bank is truly useful or not. If so, I think this type of transplantation can become standard treatment within 5 years.”
The Latest on: iPSC transplantation
[google_news title=”” keyword=”iPSC transplantation” num_posts=”10″ blurb_length=”0″ show_thumb=”left”]
via Google News
The Latest on: iPSC transplantation
- Video: Two families linked after an HIV-positive heart transplant meet for the first timeon November 24, 2022 at 3:21 pm
She's been clean for the last three decades but her kidneys began to fail and doctors at Montefiore Health System contemplated *** transplant but they determined her heart wouldn't survive the ...
- Retired nurse makes transplant history at Northwestern Medicalon November 23, 2022 at 7:48 am
CHICAGO — Surgeons have performed Northwestern Medical’s first successful combined lung-liver transplant. The patient was 63-year-old Patricio Collera who retired from nursing while working at ...
- Lung transplant for COPD patients: Patients eligible, deciding factor, benefitson November 22, 2022 at 10:38 am
Damage to the lungs can lead to a condition known as a chronic obstructive pulmonary disease (COPD) and the surgical option for those with advanced COPD is a lung transplant. According to research ...
- Kidney transplant reconnecting old elementary school friends who haven't seen each other in more than 30 yearson November 21, 2022 at 7:47 pm
"I can't describe it, it's just a true blessing." Last year, Watson shared that she was eligible for a kidney transplant, detailing her health journey in a Facebook post. She said a few friends ...
- Can You Transplant A Brain Into A Young New Body? And Would You?on November 21, 2022 at 4:00 pm
In this article, I will focus on several promising approaches for gradual brain replacement and, potentially, complete brain transplantation. 3d rendered image of Neuron cell network on black ...
- Note These Pros and Cons of Hair Transplantation Before Considering This Methodon November 19, 2022 at 9:21 am
As compared to other treatments for hair growth, transplantation is the safest and most successful with almost 99% success rate. You might get a new hairline as your new hair grows back.
- Women who met during liver transplant 35 years ago reuniteon November 18, 2022 at 5:00 pm
The organ transplant happened in 1987 when there were so many unknowns. Three decades later, they're inspiring others. Strickland and Fischman were reunited Friday, now as wives and mothers ...
- Age Bias in Allocation of Organ for Transplantationon November 18, 2022 at 1:51 am
Donor selection is an integral part of the organ transplantation program. Various factors influence the selection process in the allocation of organs for transplantation. Left digit bias ...
- Induced Pluripotent Stem Cell (iPSC) Global Market Report 2022: Use of Pluripotent Stem Cells in treating Parkinson's Disease Presents Opportunitieson November 17, 2022 at 6:15 am
Dublin, Nov. 17, 2022 (GLOBE NEWSWIRE) -- The "Induced Pluripotent Stem Cell (iPSC) Global Market Opportunities And Strategies To 2031" report has been added to ResearchAndMarkets.com's offering.
via Bing News