DNA may be the blueprint of life, but it’s also a molecule made from just a few simple chemical building blocks. Among its properties is the ability to conduct an electrical charge, fueling an engineering race to develop novel, low-cost nanoelectronic devices.
Now, a team led by ASU Biodesign Institute researcher Nongjian “N.J.” Tao and Duke theorist David Beratan has been able to understand and manipulate DNA to more finely tune the flow of electricity through it. The key findings, which can make DNA behave in different ways — cajoling electrons to smoothly flow like electricity through a metal wire, or hopping electrons about like the semiconductors materials that power our computers and cellphones — pave the way for an exciting new avenue of research advancements.
The results, published in the online edition of Nature Chemistry, may provide a framework for engineering more stable and efficient DNA nanowires, and for understanding how DNA conductivity might be used to identify gene damage.
Building on a series of recent works, the team has been able to better understand the physical forces behind DNA’s affinity for electrons.
“We’ve been able to show theoretically and experimentally that we can make DNA tunable by changing the sequence of the ‘A, T, C, or G’ chemical bases, by varying its length, by stacking them in different ways and directions, or by bathing it in different watery environments,” said Tao, who directs the Biodesign Center for Biolectronics and Biosensors and is a professor in the Ira A. Fulton Schools of Engineering.
Along with Tao, the research team consisted of ASU colleagues, including lead co-author Limin Xiang and Yueqi Li, and Duke University’s Chaoren Liu, Peng Zheng and David Beratan.
Every molecule or substance has its own unique attraction for electrons — the negatively charged particles that dance around every atom. Some molecules are selfish and hold onto or gain electrons at all costs, while others are far more generous, donating them more freely to others in need.
But in the chemistry of life, it takes two to tango. For every electron donor there is an acceptor. These different electron dance partners drive so-called redox reactions, providing energy for the majority of the basic chemical processes in our bodies.
For example, when we eat food, a single sugar molecule gets broken down to generate 24 electrons that go on to fuel our bodies. Every DNA molecule contains energy, known as a redox potential, measured in tenths of electron volts. This electrical potential is similarly generated in the outer membrane of every nerve cell, where neurotransmitters trigger electronic communication between the 100 trillion neurons that form our thoughts.
But here’s where the ability of DNA to conduct an electrical charge gets complicated. And it’s all because of the special properties of electrons — where they can behave like waves or particles due to the inherent weirdness of quantum mechanics.
Scientists have long disagreed over exactly how electrons travel along strands of DNA, said David N. Beratan, professor of chemistry at Duke University and leader of the Duke team.
“Think of trying to get across a river,” explained Limin Xiang, a postdoctoral researcher in Tao’s lab. “You can either walk across quickly on a bridge or try to hop from one rock to another. The electrons in DNA behave in similar ways as trying to get across the river, depending on the chemical information contained within the DNA.”
Previous findings by Tao (pictured left) showed that over short distances, the electrons flow across DNA by quantum tunneling that spread fast like waves across a pond. Across longer distances, they behave more like particles and the hopping takes effect.
This result was intriguing, said Duke graduate student and co-lead author Chaoren Liu, because electrons that travel in waves are essentially entering the “fast lane,” moving with more organization and efficiency than those that hop.
“In our studies, we first wanted to confirm that this wave-like behavior actually existed over longer distances,” Liu said. “And second, we wanted to understand the mechanism so that we could make this wave-like behavior stronger or extend it to longer distances.“
Flick of the switch
DNA strands are built like chains, with each link comprising one of four molecular bases whose sequence codes the genetic instructions for our cells. Like metal chains, DNA strands can easily change shape, bending, curling and wiggling around as they collide with other molecules around them.
All of this bending and wiggling can disrupt the ability of the electrons to travel like waves. Previously, it was believed that the electrons could only be shared over at most three bases.
Using computer simulations, the Beratan team found that certain sequences of bases could enhance the electron sharing, leading to wave-like behavior over long distances. In particular, they found that stacking alternating series of five guanine (G) bases created the best electrical conductivity.
The team theorizes that creating these blocks of G bases causes them to all “lock” together so the wave-like behavior of the electrons is less likely to be disrupted by the random motions of the DNA strand.
“We can think of the bases being effectively linked together so they all move as one. This helps the electron be shared within the blocks,” Liu said.
Next, the Tao group carried out conductivity experiments on short, six to 16 base strands of DNA, carrying alternating blocks of three to eight guanine bases. By tethering their test DNA between a pair of two gold electrodes, the team could flip on and control a small current to measure the amount of electrical charge flowing through the molecule.
They found that by varying a simple repeating “CxGx” pattern of DNA letters (x is the odd- or even-numbered G or C letters), there was an odd-even pattern in the ability of DNA to transport electrons. With an odd number, there was less resistance, and the electrons flowed faster and more freely (more wave-like) to blaze a path across the DNA.
They were able to exert precise molecular-level control and make the electrons hop (known as incoherent transport, the type found in most semiconductors) or flow faster (coherent transport, the type found in metals) based on variations in the DNA sequence pattern.
The experimental work confirmed the predictions of the theory.
The results shed light on a long-standing controversy over the exact nature of electron transport in DNA and might provide insight into the design of DNA nanoelectrics and the role of electron transport in biological systems, Beratan said.
In addition to practical DNA-based electronic applications (for which the group has filed several patents), one of the more intriguing aspects is relating their work — done with short simple stretches of DNA — back to the complex biology of DNA thriving inside of every cell.
Of upmost importance to survival is maintaining the fidelity of DNA to pass along an exact copy of the DNA sequence every time a cell divides. Despite many redundant protection mechanisms in the cell, sometimes things go awry, causing disease. For example, absorbing too much UV light can mutate DNA and trigger skin cancer.
One of the DNA chemical letters, “G,” is the most susceptible to oxidative damage by losing an electron (think of rusting iron — a result of a similar oxidation process). Xiang points out that long stretches of Gs are also found on the ends of every chromosome, maintained by a special enzyme known as telomerase. Shortening of these G stretches has been associated with aging.
But for now, the research team has solved the riddle of how the DNA information influences the electrical charge.
“This theoretical framework shows us that the exact sequence of the DNA helps dictate whether electrons might travel like particles, and when they might travel like waves,” Beratan said. “You could say we are engineering the wave-like personality of the electron.”
The Latest on: Tunable DNA
via Google News
The Latest on: Tunable DNA
- Chemical communications (Cambridge, England)on October 7, 2021 at 7:08 am
MicroRNA-induced cascaded and catalytic self-assembly of DNA nanostructures for enzyme-free and sensitive fluorescence detection of microRNA from tumor cells.
- Dynamic twists and loops can enable DNA to modulate its functionon September 28, 2021 at 2:02 am
When people think of DNA, they visualize a string-like double helix structure. In reality, the DNA double helix in cells is supercoiled and constrained into loops. This supercoiling and looping ...
- Aqua Cultured Foods breaks new ground in alt-seafood space with fermentation-based whole cutson September 21, 2021 at 8:00 am
‘We're not messing with its DNA sequence or altering its genetic material because we want to maintain that non-GMO status’ Unlike synthetic biology companies genetically engineering ...
- Resonant Bio-chemical Sensor Capable of Wireless Interrogationon September 14, 2021 at 7:09 pm
Biopolymers, such as Deoxyribonucleic acid (DNA)-cetyltrimethylammonium (CTMA) and bovine serum albumin (BSA), have been found to have unique dielectrical properties. These biodielectrics can exhibit ...
- SBM Life Science Reinforces Its Digitalization Effort and Automates Sales Forecasts with QAD DynaSys Cloud Solutionson September 14, 2021 at 5:11 am
"Our DNA is based, among other things, on research and innovation. This is true whether it is designing our solutions to offer the best to our customers, the technologies we employ or the processes ...
- Samsung's Exynos 2200 Phone SoC With AMD RDNA 2 Muscle Hits Benchmark Circuiton September 13, 2021 at 3:50 am
Samsung is working on a version of its Exynos system-on-chip (SoC) that will incorporate AMD's second generation Radeon DNA (RDNA 2) graphics, which is certainly exciting from both technical and ...
- Astrobiology newson September 12, 2021 at 5:00 pm
Studying DNA repair is key to future space exploration, which could expose humans to risk of DNA damage caused by radiation. Conditions in space also could affect the way the body repairs such ...
- DNA is more than its sequenceon December 15, 2020 at 11:59 am
The original view of our DNA as a relatively static B-form double helix has been disrupted by studies finding, for example, that the bases frequently and transiently adopt Hoogsteen base-pairing.
- Life Sciences A - Zon August 13, 2020 at 12:25 pm
In this interview, Professor John Rossen talks about next-generation sequencing and it's implications for the diagnosis of disease.
- Research Coreson December 1, 2019 at 12:57 am
The fitness center has aerobic and resistance machines that can be programmed to research protocols and capture exercise data. Also available are dumbbells, cable station, and balance equipment for ...
via Bing News