A novel, inexpensive method for detecting the Zika virus could help slow spread of outbreak, and potentially other future pandemic diseases
An international, multi-institutional team of researchers led by synthetic biologist James Collins, Ph.D., at the Wyss Institute for Biologically Inspired Engineering at Harvard University, has developed a low-cost, rapid paper-based diagnostic system for strain-specific detection of the Zika virus, with the goal that it could soon be used in the field to screen blood, urine, or saliva samples.
“The growing global health crisis caused by the Zika virus propelled us to leverage novel technologies we have developed in the lab and use them to create a workflow that could diagnose a patient with Zika, in the field, within 2-3 hours,” said Collins, who is a Wyss Core Faculty member, and Termeer Professor of Medical Engineering & Science and Professor of Biological Engineering at the Massachusetts Institute of Technology (MIT)’s Department of Biological Engineering.
Building off previous work done at Harvard’s Wyss Institute by Collins and his team, along with collaborators from Massachusetts Institute of Technology (MIT), the Broad Institute of Harvard and MIT, Harvard Medical School (HMS), University of Toronto, Arizona State University (ASU), University of Wisconsin-Madison (UW-Madison), Boston University (BU), Cornell University, and Addgene, joined their efforts to quickly prototype the rapid diagnostic test and describe their methods in a study published online May 6 in the journal Cell, all within a matter of six weeks. Collins is the paper’s corresponding author.
Emerging innovation during the Ebola health crisis
In October 2014, Collins’ team developed a breakthrough method for embedding synthetic gene networks — which could be used as programmable diagnostics and sensors – on portable, small discs of ordinary paper.
Stirred by the then-ongoing Ebola outbreak in Africa, they demonstrated a proof-of-concept color-changing diagnostic that could screen for Ebola by embedding in paper a novel kind of synthetic biomolecular sensor designed to screen for specific RNA sequences. These RNA sequences can mark not only the genetic signatures of Ebola but also other RNA viruses including Zika, SARS, measles, influenza, hepatitis C, and West Nile fever. The team believed that one day, the method could be applied in the field to identify viruses with blood, urine, or saliva samples.
However, until recently, the team’s paper-based technology has been challenged by the extremely low concentration of virus that is normally found in blood, urine, and saliva. Now, using blood samples from monkeys infected with Zika virus as well as virus recovered from cells infected in the laboratory, the team has validated a next generation technique that overcomes this problem.
A leap for paper-based diagnostics, urged by the Zika outbreak
“The vivid images in the news stemming from the ongoing Zika crisis are heartbreaking,” said Keith Pardee, Ph.D., one of the study’s co-first authors and an Assistant Professor in the Leslie Dan Faculty of Pharmacy at University of Toronto, who was formerly a Postdoctoral Fellow at the Wyss Institute and BU. “We hope a tool like this can help reduce the impact of the outbreak until a vaccine can be developed.”
With field use in mind, Collins’ team designed a simple modular workflow comprising three steps: amplification, Zika detection, and CRISPR-Cas9-aided strain identification. CRISPR-Cas9, a gene editing mechanism derived from the immune systems of bacteria, can be used to search entire sequences to find exclusive genetic markers. Leveraging CRISPR-Cas9’s talent for sequence recognition, the third part of the team’s system uses a CRISPR-Cas9-aided paper-based diagnostic to discriminate between strains whose genetic profiles differ by as little as one nucleotide.
Learn more: Finding Zika one paper disc at a time
The Latest on: Zika
via Google News
The Latest on: Zika
- Zika Virus Vaccines Market Share, Growth Forecast- Global Industry Outlook 2025on May 23, 2022 at 12:30 am
With the zika virus spreading at an alarming rate across the world, the global market for zika virus vaccines is likely to gain substantial momentum across the world. The recent outbreak of the viral ...
- Does anyone know what the situation with Zika is...on May 22, 2022 at 2:07 pm
Concerned about zika request a pool side room, non existent..also like Big red stated they spray twice daily down the access road but it drives the bugs onto the "jungle side porches" ...
- Zika Virus Testing Market Growth, Trends, And Forecasts (2022 2028)on May 19, 2022 at 6:47 pm
(MENAFN- Ameliorate Solutions) Posted on May 19, 2022 by Viviana Jackson Zika Virus Testing Market – Growth, Trends, And Forecasts (2022 – 2028) MarketIntelligenceData recently published a ...
- Federal Agencies’ Rampant Incompetence Fuels Zika Outbreakon May 19, 2022 at 5:00 pm
In the Sussmann Trial, What a Difference a Text Makes California’s Government Goes to War against Private Business Senate Was Right to Pass Ukraine-Aid Bill Ted Cruz Answers the Ukraine-Aid Bill ...
- Mosquitos that could carry Zika found in Des Moines and Lee counties, but none have tested positive for the viruson May 19, 2022 at 4:22 pm
Zika virus can be transmitted to humans via bites from infected mosquitoes. The most common symptoms of the disease caused by the virus are generally mild and include fever, rash, conjunctivitis ...
- Mosquitos that could carry Zika found in Des Moines and Lee counties, but none have tested positive for the viruson May 19, 2022 at 7:31 am
A collaborative study between Iowa State University, the Iowa Department Public Health and Des MoinesCounty Public Health has led to the discovery that a species of mosquitos capable of carrying the ...
- Zika Virus Testing Market – Growth, Trends, And Forecasts (2022 – 2028)on May 19, 2022 at 4:10 am
MarketIntelligenceData recently published a research report called Global Zika Virus Testing Market. Primary and secondary research methods were used to ...
- Zika Virus Vaccine Market Potential Growth, Share, Demand and Analysis of Key Players & Forecasts to 2030on May 18, 2022 at 1:21 pm
Global Zika Virus Vaccine Market report emphasizes on the detailed understanding of some decisive factors such as size, share, sales, forecast trends, supply, production, demands, industry and CAGR in ...
- For over four months, health authorities have taken no action on Zika infection cases in Indiaon May 14, 2022 at 5:00 pm
Indian government, despite reporting three laboratory-confirmed cases of Zika virus infection to World Health Organization, kept mum about the detection of Zika infection in India. Zika virus is ...
- Zika Virus Vaccines Size, Growth, Opportunity and Forecast Till 2022-2031on May 13, 2022 at 3:45 pm
Japan, Japan, Fri, 13 May 2022 04:58:27 / Comserve Inc. / -- Zika Virus Vaccines Size by Type, By Distribution Channel, And Forecast till 2022-2031 It is predicted that Zika virus vaccines shot ...
via Bing News