
Scanning electron microscope images of a normal amphipod (top), about one millimeter long, with color-coded hind jumping legs, and an amphipod that has been genetically modified with CRISPR to grow forward-walking legs instead (bottom). (Arnaud Martin image)
The simplicity of CRISPR-Cas9 gene editing will soon make studying the genes of any organism, from the simplest slime mold to the octopus, as easy as it now is to study the genes controlling development in standard lab animals such as nematodes, fruit flies, frogs and mice.
A new study from UC Berkeley illustrates the ease with which CRISPR-Cas9 can knock out genes in exotic animals — in this case, an amphipod or sandhopper — to learn how those genes control growth and development. Researchers wanted to know which genes control the development of appendages on each segment of the amphipod, whose body is like a Swiss army knife with each segment bearing a different blade or tool as an appendage.
In less time than it would have taken two years ago to knock out one gene in the animal, UC Berkeley researchers knocked out six, shedding light on the basic genetic mechanisms that determine leg anatomy in the evolution of animals. By knocking out, one by one, the so-called Hox genes that specify body parts in all animals, they switched the identities of the crustacean’s limbs, transforming a claw into a leg, for example, or a jaw into an antenna.
“For those of us studying non-traditional model organisms in the lab, this has the flavor of a technical revolution,” said UC Berkeley postdoctoral researcher Arnaud Martin. “CRISPR-Cas9 definitely changes the range of possibilities in working with exotic animals, and the amphipod is one of the first.”
The Hox family of genes Martin is investigating is found in all animals, and some biologists argue that an animal can be defined as any creature having Hox genes. They are known to turn on or off a myriad of genes in each segment of the body. Humans’ body segments are most evident in the spine, where each type of spinal vertebra is unique in shape, all under the control of Hox genes.
In the tropical marine amphipod Parhyale hawaiensis, Martin and Patel wanted to know which of the Hox genes function in each of its 19 segments to produce mouthparts at the front, then claws, followed by forward-moving legs, powerful jumping legs that propel the crustacean backwards, swimming legs and finally stumpy “anchor” legs.
“CRISPR-Cas9 has made a huge impact already in model systems, like the fruit fly Drosophila and the nematode C. elegans, where there are already a lot of tools. People were able to incorporate the technology very quickly and change the way they make mutants in those animals,” said senior author Nipam Patel, a UC Berkeley professor of molecular and cell biology and of integrative biology. “But there are a lot of other animals we want to work with because we can use them to answer really basic evolutionary questions. CRISPR-Cas9 is a great technology for that.”
Other researchers have recently used CRISPR-Cas9 to edit genes in unusual organisms like the sea anemone, the lamprey and butterfly to see whether the tool works, but for the most part these have been “proof-of-principle” experiments using CRISPR-Cas9 to confirm what was already known.
“Here we have gone way beyond just showing that it works in Parhyale and jumped right into answering a really important developmental and evolutionary question: how you set up all of these limbs in the crustacean, and how evolution changes that pattern of limbs by altering Hox gene expression patterns,” Patel said.
Read more: CRISPR-Cas9 helps uncover genetics of exotic organisms
The Latest on: CRISPR-Cas9 gene editing
via Google News
The Latest on: CRISPR-Cas9 gene editing
- TALEN outperforms Cas9 in editing heterochromatin target siteson January 27, 2021 at 3:37 am
While Cas9 outperforms TALENs in euchromatin, it is less efficient in heterochromatic regions. Here the authors, using single-molecule imaging, show that Cas9 uses a less efficient search strategy ...
- Spotting Every Needle in the Haystack: CRISPeR Gene Editing for DNA with Lone Mutationson January 25, 2021 at 5:36 am
The quest to change the very nature of being, has driven philosophical and metaphysical debates for ages. Now, with the advent of ...
- Gene editing techniques helpful in retinal degeneration treatmenton January 23, 2021 at 9:30 pm
A new study has found that gene-editing techniques like CRISPR-Cas hold the power to rectify inherited retinal degenerative mutations, which are the primary cause of blindness.
- Researcher expands plant genome editing with newly engineered variant of CRISPR-Cas9on January 22, 2021 at 9:46 am
Alongside Dennis van Engelsdorp, associate professor at the University of Maryland (UMD) in Entomology named for the fifth year in a row for his work in honey bee and pollinator health, Yiping Qi, ...
- Gene Editing Technique Halts Premature Aging In Miceon January 21, 2021 at 12:41 pm
A specialized gene editing technique that allows very small and precise changes to be made to the genetic code can halt a disease that causes premature aging in a mouse model and double the lifespan ...
- Chinese scientists develop gene therapy that could delay ageingon January 21, 2021 at 12:51 am
A new gene therapy has been shown to reverse some of the effects of ageing in mice and extend their lifespans by up to a quarter. Chinese researchers used CRISPR-Cas9, a powerful gene-editing tool, to ...
- Plant genome editing expanded with newly engineered variant of CRISPR-Cas9on January 20, 2021 at 4:00 pm
Scientists have created a newly engineered variant of the famed gene editing tool CRISPR-Cas9. SpRY removes the barriers of what can and can't be targeted for gene editing, making it possible for the ...
- Gene-editing 'scissor' tool may also be a 'dimmer switch'on January 19, 2021 at 10:48 am
offers opportunities to design new or better CRISPR-Cas9 tools aimed at regulating gene activity for research purposes. "In a gene editing scenario, a researcher may want to cut a specific gene ...
- CRISPR technology shown to dial down gene activity in bacteriaon January 19, 2021 at 7:31 am
In a series of experiments with laboratory-cultured bacteria, Johns Hopkins scientists have found evidence that there is a second role for the widely used gene-cutting system CRISPR-Cas9—as a genetic ...
- Gene editing techniques helpfulon January 15, 2021 at 5:00 am
A new study has found that gene-editing techniques like CRISPR-Cas hold the power to rectify inherited retinal degenerative mutations which are the primary cau ...
via Bing News