Findings can lead to gut replacement therapy in people with intestinal deficiencies
Working with gut stem cells from humans and mice, scientists from the Johns Hopkins Children’s Center and the University of Pittsburgh have successfully grown healthy intestine atop a 3-D scaffold made of a substance used in surgical sutures.
In a further step that takes their work well beyond proof of concept, researchers report their laboratory-created intestine successfully regenerated gut tissue in the colons of dogs with missing gut lining.
The experiments, described ahead of print in the journal Regenerative Medicine, bring researchers closer to creating an implantable intestine as replacement therapy for a range of devastating disorders – including infections, cancer and trauma – that result in loss or death of gut tissue. Chief among them is a condition that affects 12 percent of premature newborns, called necrotizing enterocolitis (NEC), which is marked by the rapid death of intestinal cells and permanent loss of intestinal tissue.
The tube-shaped scaffold, designed several years ago in collaboration with Cornell University researchers and composed of biodegradable material similar to that used in surgical sutures, was a big first step on the quest to develop an implantable replacement intestine. But the new work pushes that effort further because it shows how stem cells, when mixed with immune and connective tissue cells, can grow into normal gut tissue around the scaffold and function inside a living mammal.
Researchers caution that a fully functioning replacement intestine for humans is far off, but they say their results have laid the critical groundwork to do so.
“Our experiments show that the architecture and function of our lab-made intestine strikingly resemble those of the healthy human gut, giving us real hope that our model could be used as the backbone for replacement intestine,” says principal investigator David Hackam, M.D., Ph.D., the Johns Hopkins Children’s Center’s surgeon-in-chief, who initiated and conducted most of the work at the University of Pittsburgh.
In an initial set of experiments reminiscent of a peanut butter-and-jelly sandwich technique, researchers took stem cells from the colons of babies undergoing intestinal surgeries and from mice, then added immune cells called macrophages, the body’s scavengers that seek out and engulf debris along with foreign and diseased cells. To this mix, they added cells called fibroblasts, whose function is to form collagen and other connective substances that bind tissues and organs together. The idea, the scientists say, was to create a mixture that closely mimics the natural composition of the gut.
“Intestinal cells do not grow and develop in a vacuum, so we sought to recreate the richness of the human gut, Hackam says. “We took the PB & J approach, adding a layer of stem cells on top of the scaffold, then topping them with a mixture of immune and collagen cells.”
Adding these components, they report, enhanced the growth of intestinal stem cells and differentiation into various mature cell types critical to the function of a healthy intestine. In comparison, stem cells grown in isolation without immune and connective cells in the mix grew more slowly and failed to differentiate well into multiple cell types.
In another set of experiments, researchers added probiotic bacteria to the newly created intestinal tissue. Doing so further amplified the growth and differentiation of new gut cells, specifically the growth of Paneth cells responsible for production of infection-fighting proteins that guard against intestinal infections, Hackam says, a finding that highlights the therapeutic potential of certain probiotics for NEC. Next, the team added bacteria-laden stool cells obtained from infants with NEC, but they observed a dramatic decrease in the number of intestinal cells called goblet cells, which are responsible for the production of protective mucus that coats and shields the intestine – a common occurrence in NEC. The observation, Hackam says, supports the notion that certain gut bacteria present in the colons of babies with NEC are responsible for sparking the rapid death of gut tissue that occurs in the condition.
Next, researchers implanted the newly created intestine into the bellies of mice. In a matter of days, the implanted intestine began producing new intestinal stem cells and stimulated the growth of new blood vessels around the implant. That observation, researchers say, affirmed the ability of the 3-D intestine to spur the growth of new tissue not only in lab dishes, but also in living organisms.
In a final step, the investigators implanted pieces of the newly created intestine – about 1.6 inches in length – into the lower portion of dog colons lacking parts of their intestinal lining. For two months, the dogs underwent periodic colonoscopies and intestinal biopsies. Strikingly, the guts of dogs with implanted intestines healed completely within eight weeks. By contrast, dogs that didn’t get intestinal implants experienced continued inflammation and scarring of their guts.
The Latest on: Regenerative medicine
via Google News
The Latest on: Regenerative medicine
- APEX Biologix Launches its Newly Reconfigured Bone Marrow System for Regenerative Medicine Procedureson May 17, 2022 at 7:00 am
XCELL BMC to Provide System Features Specifically Designed for Surgery Settings in Hospitals, Clinics, and Surgical Centers ...
- QC Kinetix (Lubbock) Offers Pain Treatment Through Regenerative Medicine in Lubbock, TXon May 17, 2022 at 6:36 am
Lubbock, TX - QC Kinetix (Lubbock) uses regenerative medicine and biologic therapies to treat pain caused by different conditions without the patient ...
- QC Kinetix (Longview) Unveils Regenerative Medicine as an Alternative to Surgery for Sports Injuries and Chronic Pain in Longview, TXon May 17, 2022 at 6:35 am
There are many benefits associated with regenerative medicine. For starters, it’s a great option to treat pain due to arthritis in areas such as knees and hips without undergoing surgery. The ...
- Pain Defying Regenerative Medicine Treatment at QC Kinetix (Springs Medical) in Louisville, KYon May 17, 2022 at 6:20 am
With the new regenerative medicine at QC Kinetix (Springs Medical), the residents of Louisville, KY, have access to pain control providers keen to deliver lasting relief for chronic pain. Regenerative ...
- How QC Kinetix (Augusta) is Winning Hearts Thanks to its Regenerative Medicine Treatments in Augusta, GAon May 13, 2022 at 1:00 pm
QC Kinetix (Augusta) understands the gravity of chronic pain on the quality of life and is committed to providing regenerative medicine as a countermeasure. The medical team offers treatment for pain ...
- PolarityTE Announces FDA Regenerative Medicine Advanced Therapy Designation Granted to SkinTE®on May 13, 2022 at 5:00 am
PolarityTE, Inc. (Nasdaq: PTE) today announced that the U.S. Food and Drug Administration (FDA) granted a Regenerative Medicine Advanced Therapy (RMAT) designation to SkinTE under the Company's open ...
- University Of Colorado To Invest $200 Million In New Regenerative Medicine Instituteon May 12, 2022 at 4:49 am
The University of Colorado Anschutz Medical Campus has announced that it invest $200 million over the next five years to create the Gates Institute, a research and treatment center that will focus on ...
- $83 Bn Regenerative Medicine Markets - Global Opportunity Analysis and Industry Forecast, 2021-2022 & 2030 - ResearchAndMarkets.comon May 11, 2022 at 6:29 am
The “Regenerative Medicine Market by Product Type, Material, Application and End user (Hospitals, Ambulatory Surgical Centers, and Others: Global Opportunity Analysis and Industry Forecast, 2021-2030” ...
- Orthobiologics: how regenerative medicine can make a difference in orthopedic surgeryon May 5, 2022 at 8:07 am
Regenerative medicine is a new field of medicine in which orthopedic surgeons aim to move away from replacement and toward joint preservation ...
- Regenerative Medicine Market: By Key Players, Market Size, Deployment Type, Applications, Vertical, and Forecast: 2022-2031on April 27, 2022 at 8:59 pm
Japan, Japan, Thu, 28 Apr 2022 03:57:48 / Comserve Inc. / -- Global Regenerative Medicine Market was valued at US$ 29.1 billion in 2031 and is anticipated to reach US$ 178.6 billion by 2031 ...
via Bing News